Sakurai A, Ichikawa K, Hashizume K, Miyamoto T, Yamauchi K, Ohtsuka H, Nishii Y, Yamada T
Department of Gerontology, Endocrinology, and Metabolism, Shinshu University School of Medicine, Matsumoto, Japan.
J Endocrinol. 1989 May;121(2):337-41. doi: 10.1677/joe.0.1210337.
The effects of histone subfractions on rat liver thyroid hormone receptor-DNA interaction were examined using an in-vitro DNA-cellulose binding assay. H1 histones bound to DNA showed reversible and potent inhibition of receptor-DNA binding without affecting receptor hormone binding. Poly-lysine, bovine serum albumin, ovalbumin and cytochrome c did not alter receptor-DNA binding. H1 histone subfractions (calf thymus lysine-rich histone (CTL)-1, CTL-2 and CTL-3) showed potent inhibition of receptor-DNA binding indistinguishable from each other. The quantity of H1 histone subfractions bound to DNA was the same. Although each subfraction has different functional properties, inhibition of receptor-DNA binding was a common feature of all the H1 histone subfractions, which is important for the non-random distribution of the receptor in chromatin. Binding of the receptor to core histones was investigated; it was found to bind to core histones more potently than to other proteins (H1 histone, ovalbumin and cytochrome c). Among core histone subfractions, H4 histone bound to the receptor most potently and is the candidate to be one of the acceptor sites of the receptor in chromatin.
使用体外DNA-纤维素结合试验检测了组蛋白亚组分对大鼠肝脏甲状腺激素受体与DNA相互作用的影响。与DNA结合的H1组蛋白对受体与DNA的结合表现出可逆且强效的抑制作用，而不影响受体与激素的结合。聚赖氨酸、牛血清白蛋白、卵清蛋白和细胞色素c均未改变受体与DNA的结合。H1组蛋白亚组分（小牛胸腺富含赖氨酸的组蛋白（CTL）-1、CTL-2和CTL-3）对受体与DNA的结合表现出强效抑制作用，彼此之间无明显差异。与DNA结合的H1组蛋白亚组分的量相同。尽管每个亚组分具有不同的功能特性，但抑制受体与DNA的结合是所有H1组蛋白亚组分的共同特征，这对于受体在染色质中的非随机分布很重要。研究了受体与核心组蛋白的结合；发现它与核心组蛋白的结合比与其他蛋白质（H1组蛋白、卵清蛋白和细胞色素c）的结合更强效。在核心组蛋白亚组分中，H4组蛋白与受体的结合最有效，是染色质中受体的受体位点之一的候选者。
