Skinner M A, Racaniello V R, Dunn G, Cooper J, Minor P D, Almond J W
Department of Microbiology, University of Reading, England.
J Mol Biol. 1989 May 20;207(2):379-92. doi: 10.1016/0022-2836(89)90261-1.
A secondary structure model for the 5' non-coding RNA of poliovirus has been derived by comparing computer-generated folding patterns of equivalent sequences from a number of related enteroviruses and rhinoviruses and identifying compensating mutations that suggest conservation of a common secondary structure. Although certain elements are similar, the new model differs considerably from a previously published minimal energy structure and is consistent with the observed sensitivity of in vitro RNA transcripts of infectious poliovirus cDNA to RNases and modifying chemicals. The sequence of a neurovirulent revertant of an attenuated mutant provides additional evidence for an interaction between a region known to be important for neurovirulence, sequence 471-483, and nucleotides 528 to 538.
通过比较多种相关肠道病毒和鼻病毒的等效序列的计算机生成折叠模式，并识别表明共同二级结构保守性的补偿性突变，得出了脊髓灰质炎病毒5'非编码RNA的二级结构模型。尽管某些元件相似，但新模型与先前发表的最小能量结构有很大不同，并且与感染性脊髓灰质炎病毒cDNA的体外RNA转录本对核糖核酸酶和化学修饰剂的敏感性一致。减毒突变体的神经毒力回复株的序列为已知对神经毒力重要的区域（序列471 - 483）与核苷酸528至538之间的相互作用提供了额外证据。
