Kim Hangeun, Kim Hye Rim, Kim Na-Ra, Jeong Bong Jun, Lee Jong Suk, Jang Soojin, Chung Dae Kyun
RNA Inc., #308 College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
J Microbiol. 2015 Jan;53(1):47-52. doi: 10.1007/s12275-015-4483-z. Epub 2014 Dec 4.
Lactobacillus plantarum is a well-documented probiotic that has been used in clinical trials for the regulation of the immune system and treatment of gastrointestinal diseases. In this study, we evaluated the effects of L. plantarum cell lysates on the immune regulation through the in vitro and in vivo studies. L. plantarum lysates were prepared by sonication method, and we observed that the repetition of disruption step increased indicator components within the bacterial lysates. Indicator components might affect TNF-α production. L. plantarum lysates did not induce TNF-α production, while LPS-induced TNF-α production was dramatically inhibited in a sonication-dependent manner in THP-1 cells. Oral administration of L. plantarum lysates effectively attenuated the horny layer formation and decreased epidermal thickening in NC/Nga mice skin. The damage to barrier function after the 8 weeks oral administration was reduced by L. plantarum lysates as compared to that in the atopic dermatitis (AD) mice. Further study revealed that L. plantarum lysates polarized Th1 response via induction of IL-12 and IFN-γ production and inhibition of IL-4 and IgE production in NC/Nga mice. Together, our results suggest that L. plantarum lysates are remarkable material for host homeostasis and it could be used for the treatment of inflammatory diseases.
植物乳杆菌是一种有充分文献记载的益生菌,已用于免疫系统调节和胃肠道疾病治疗的临床试验。在本研究中,我们通过体外和体内研究评估了植物乳杆菌细胞裂解物对免疫调节的影响。植物乳杆菌裂解物采用超声破碎法制备,我们观察到破碎步骤的重复增加了细菌裂解物中的指示成分。指示成分可能会影响肿瘤坏死因子-α(TNF-α)的产生。植物乳杆菌裂解物不会诱导TNF-α的产生,而在THP-1细胞中,脂多糖(LPS)诱导的TNF-α产生以超声依赖的方式受到显著抑制。口服植物乳杆菌裂解物可有效减轻NC/Nga小鼠皮肤角质层形成并减少表皮增厚。与特应性皮炎(AD)小鼠相比,口服8周后,植物乳杆菌裂解物可减轻屏障功能损伤。进一步研究表明,植物乳杆菌裂解物通过诱导NC/Nga小鼠产生白细胞介素-12(IL-12)和干扰素-γ(IFN-γ)以及抑制IL-4和免疫球蛋白E(IgE)的产生,使Th1反应极化。总之,我们的结果表明,植物乳杆菌裂解物是维持宿主稳态的重要物质,可用于治疗炎症性疾病。