Lee Hae Mi, Lee Seungok, Lee Bo-In, Jekarl Dong Wook, Song Joo-Yong, Choi Hye-Jung, Kang Bong Koo, Im Eun Joo, Kim Joon Sung, Kim Jong In, Kim Byung-Wook, Choi Hwang
Department of Internal Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea.
Department of Laboratory Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea College of Medicine, Incheon, Korea.
Gut Liver. 2015 Sep 23;9(5):636-40. doi: 10.5009/gnl14106.
BACKGROUND/AIMS: The diagnostic yield of fecal leukocyte and stool cultures is unsatisfactory in patients with acute diarrhea. This study was performed to evaluate the clinical significance of the fecal lactoferrin test and fecal multiplex polymerase chain reaction (PCR) in patients with acute diarrhea.
Clinical parameters and laboratory findings, including fecal leukocytes, fecal lactoferrin, stool cultures and stool multiplex PCR for bacteria and viruses, were evaluated prospectively for patients who were hospitalized due to acute diarrhea.
A total of 54 patients were included (male, 23; median age, 42.5 years). Fecal leukocytes and fecal lactoferrin were positive in 33 (61.1%) and 14 (25.4%) patients, respectively. Among the 31 patients who were available for fecal pathogen evaluation, fecal multiplex PCR detected bacterial pathogens in 21 patients, whereas conventional stool cultures were positive in only one patient (67.7% vs 3.2%, p=0.000). Positive fecal lactoferrin was associated with presence of moderate to severe dehydration and detection of bacterial pathogens by multiplex PCR (21.4% vs 2.5%, p=0.049; 100% vs 56.5%, p=0.032, respectively).
Fecal lactoferrin is a useful marker for more severe dehydration and bacterial etiology in patients with acute diarrhea. Fecal multiplex PCR can detect more causative organisms than conventional stool cultures in patients with acute diarrhea.
背景/目的：急性腹泻患者粪便白细胞和粪便培养的诊断率并不理想。本研究旨在评估粪便乳铁蛋白检测和粪便多重聚合酶链反应（PCR）在急性腹泻患者中的临床意义。
对因急性腹泻住院的患者前瞻性评估临床参数和实验室检查结果，包括粪便白细胞、粪便乳铁蛋白、粪便培养以及针对细菌和病毒的粪便多重PCR。
共纳入54例患者（男性23例；中位年龄42.5岁）。粪便白细胞和粪便乳铁蛋白阳性患者分别为33例（61.1%）和14例（25.4%）。在31例可进行粪便病原体评估的患者中，粪便多重PCR检测到21例患者有细菌病原体，而传统粪便培养仅1例阳性（67.7%对3.2%，p = 0.000）。粪便乳铁蛋白阳性与中重度脱水的存在以及多重PCR检测到细菌病原体相关（分别为21.4%对2.5%，p = 0.049；100%对56.5%，p = 0.032）。
粪便乳铁蛋白是急性腹泻患者更严重脱水和细菌病因的有用标志物。在急性腹泻患者中，粪便多重PCR比传统粪便培养能检测到更多致病生物。
Zotero 插件