Department of Medical Microbiology, Cork University Hospital, Wilton, Cork, Ireland.
J Clin Microbiol. 2009 Nov;47(11):3449-53. doi: 10.1128/JCM.01026-09. Epub 2009 Sep 2.
The EntericBio system uses a multiplex PCR assay for the simultaneous detection of Campylobacter spp., Salmonella enterica, Shigella spp., and Escherichia coli O157 from feces. It combines overnight broth enrichment with PCR amplification and detection by hybridization. An evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative PCR detection methods. In a study of 773 samples, routine culture and the EntericBio system yielded 94.6 and 92.4% negative results, respectively. Forty-two samples had positive results by culture, and all of these were positive with the EntericBio system. This system detected an additional 17 positive samples (Campylobacter spp., n = 12; Shigella spp., n = 1; E. coli O157, n = 4), but the results for 5 samples (Campylobacter spp., n = 2; Shigella spp., n = 1; E. coli O157, n = 2) could not be confirmed. The target for Shigella spp. detected by the EntericBio system is the ipaH gene, and the molecular indication of the presence of Shigella spp. was investigated by sequence analysis, which confirmed that the ipaH gene was present in a Klebsiella pneumoniae isolate from the patient. The sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 99.3%, 91.5%, and 100%, respectively. Turnaround times were significantly reduced with the EntericBio system, and a result was available between 24 and 32 h after receipt of the sample in the laboratory. In addition, the amount of laboratory waste was significantly reduced by use of this system. In summary, the EntericBio system proved convenient to use, more sensitive than the conventional culture used in this study, and highly specific; and it generated results significantly faster than routine culture for the pathogens tested.
肠道生物系统使用多重 PCR 检测法同时从粪便中检测弯曲菌属、沙门氏菌属、志贺氏菌属和大肠杆菌 O157。它将过夜肉汤富集与 PCR 扩增和杂交检测相结合。通过将该系统的结果与常规培养的结果进行比较，并辅以替代 PCR 检测方法，对该系统进行了评估。在一项对 773 个样本的研究中，常规培养和肠道生物系统的阴性结果率分别为 94.6%和 92.4%。42 个样本经培养呈阳性，所有这些样本均经肠道生物系统呈阳性。该系统还检测到 17 个额外的阳性样本（弯曲菌属，n = 12；志贺氏菌属，n = 1；大肠杆菌 O157，n = 4），但其中 5 个样本（弯曲菌属，n = 2；志贺氏菌属，n = 1；大肠杆菌 O157，n = 2）的结果无法得到确认。肠道生物系统检测到的志贺氏菌属的靶标是 ipaH 基因，通过序列分析研究了存在志贺氏菌属的分子指标，证实了患者分离的肺炎克雷伯菌中存在 ipaH 基因。灵敏度、特异性、阳性预测值和阴性预测值分别为 100%、99.3%、91.5%和 100%。使用肠道生物系统显著缩短了周转时间，在实验室收到样本后的 24 至 32 小时内即可获得结果。此外，该系统显著减少了实验室废物的产生。总之，肠道生物系统使用方便，比本研究中使用的常规培养更敏感，特异性更高；并且与常规培养相比，该系统产生结果的速度明显更快。
