Department of Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Int J Stem Cells. 2014 Nov;7(2):87-97. doi: 10.15283/ijsc.2014.7.2.87.
Variety of pathological factors including viral hepatitis, alcohol and drug abuse, metabolic diseases, autoimmune diseases and congenital abnormalities can cause hepatic injury. Liver transplantation is the treatment of choice for end-stage liver diseases, however, it faces several difficulties. So the aim of the work is to evaluate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the liver structure in carbon tetra chloride CCL4 induced liver fibrosis in rats.
BM-MSCs were isolated and characterized from long bones of twenty male albino rats. Sixty female rats were divided into the following two groups: Group I; thirty rats which were the control group. Group II; thirty rats were injected intra-peritoneal (IP) by CCL4 twice weekly for four weeks and was further subdivided into the following three subgroups: Subgroup IIA (CCL4 alone); included ten rats which were sacrificed after this four weeks. Subgroup IIB (CCL4/MSCs); included ten rats which were IP injected by a single dose of BM-MSCs and were sacrificed after four weeks. Subgroup IIC (CCL4/recovery); included ten rats which were left for another four weeks without any intervention. Histological examination of liver specimens showed that CCl4 caused variable pathological changes with elevated liver enzymes. Injection of BM-MSCs revealed an improvement in the histological picture of the liver and its enzymatic profile. On the other hand, most of the pathological lesion were still detected in rats of recovery group.
BM-MSC could restore the liver structure and function in experimental model of liver fibrosis.
多种病理因素，包括病毒性肝炎、酒精和药物滥用、代谢性疾病、自身免疫性疾病和先天性异常，均可导致肝损伤。肝移植是治疗终末期肝病的首选方法，但面临许多困难。因此，本研究旨在评估骨髓间充质干细胞（BM-MSCs）对四氯化碳（CCL4）诱导的大鼠肝纤维化模型中肝脏结构的影响。
从 20 只雄性白化大鼠的长骨中分离和鉴定 BM-MSCs。将 60 只雌性大鼠分为以下两组：第 I 组，30 只大鼠为对照组。第 II 组，30 只大鼠每周两次腹腔内注射 CCL4 共 4 周，并进一步分为以下三个亚组：亚组 IIA（CCL4 单独），10 只大鼠在这 4 周后处死。亚组 IIB（CCL4/MSCs），10 只大鼠单次注射 BM-MSCs 后处死。亚组 IIC（CCL4/恢复），10 只大鼠在没有任何干预的情况下再观察 4 周。肝组织学检查显示，CCL4 导致肝脏酶升高的各种病理变化。BM-MSCs 注射显示肝脏组织学和酶谱均有改善。另一方面，在恢复组大鼠中仍检测到大多数病理病变。
BM-MSC 可恢复肝纤维化模型中肝脏的结构和功能。
