1,25-二羟基维生素D3受体调控启动子的计算机分析：鉴定候选D3反应元件。

Computer analysis of 1,25-dihydroxyvitamin D3-receptor regulated promoters: identification of a candidate D3-response element.

作者信息

Minghetti P P, Gibbs P E, Norman A W

机构信息

Department of Biochemistry, University of California, Riverside 92521.

出版信息

Biochem Biophys Res Commun. 1989 Jul 31;162(2):869-75. doi: 10.1016/0006-291x(89)92390-5.

DOI:10.1016/0006-291x(89)92390-5

PMID:2547377

Abstract

The receptor for the steroid hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] belongs to a superfamily of steroid and triidothyronine (T3) receptors. As yet no 1,25(OH)2D3 response element (DRE) has been identified. Since the T3 and 1,25(OH)2D3 receptors are structurally homologous, we have used the nucleotide sequence of the T3 response element to carry out a computer search of the promoter region of several 1,25(OH)2D3 regulated genes. We report here one candidate DRE from the chick calbindin-D28K gene (AGCCCAATGGCTGAACA) and two candidate DRE's from the rat osteocalcin gene (TCCCCACTGGATGAGCG and CCTGCACTGGGTGAATGA).

摘要

类固醇激素1,25 - 二羟基维生素D3 [1,25(OH)2D3] 的受体属于类固醇和三碘甲状腺原氨酸 (T3) 受体超家族。目前尚未鉴定出1,25(OH)2D3反应元件 (DRE)。由于T3和1,25(OH)2D3受体在结构上具有同源性，我们利用T3反应元件的核苷酸序列对几个1,25(OH)2D3调节基因的启动子区域进行了计算机搜索。我们在此报告来自鸡钙结合蛋白-D28K基因的一个候选DRE (AGCCCAATGGCTGAACA) 和来自大鼠骨钙素基因的两个候选DRE (TCCCCACTGGATGAGCG和CCTGCACTGGGTGAATGA)。