Song Zhenfeng, Wang Huan, Zhu Lin, Han Mingbao, Gao Yuan, Du Yu, Wen Ying
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Road, Nangang District, 150086, Harbin, People's Republic of China.
Food Funct. 2015 Feb;6(2):461-9. doi: 10.1039/c4fo00608a.
Curcumin is a yellow pigment isolated from Corcuma longan. This research investigates the improvement of curcumin on INS-1 cells with insulin resistance induced by high glucose. INS-1 cells were treated with high glucose (30 mmol L(-1)) for 48 h. Subsequently, the medium was replaced with curcumin for 24 h. Curcumin effectively increased insulin gene expression and glucose stimulated insulin secretion (GSIS) in a dose-dependent manner. Furthermore, the molecular mechanism of curcumin-induced insulin expression and secretion in high glucose-induced INS-1 cells was investigated in this study. Curcumin increased the expression of glucose transporter 2 (GLUT2) and phosphorylation of insulin receptor (IR), insulin receptor substrate-1 (IRS1), phosphatidylinositol-3-kinase (PI3K) and AKT in the INS-1 cells. Moreover, curcumin stimulation increased the expression of PDX-1 and GCK. This investigation suggests that curcumin prevented high glucose-reduced insulin expression and secretion through activation of the PI3K/Akt/GLUT2 pathway in INS-1 cells.
姜黄素是从姜黄中分离出的一种黄色色素。本研究探讨姜黄素对高糖诱导的胰岛素抵抗INS-1细胞的改善作用。将INS-1细胞用高糖（30 mmol L(-1)）处理48小时。随后，更换为含姜黄素的培养基处理24小时。姜黄素以剂量依赖的方式有效增加胰岛素基因表达和葡萄糖刺激的胰岛素分泌（GSIS）。此外，本研究还探讨了姜黄素在高糖诱导的INS-1细胞中诱导胰岛素表达和分泌的分子机制。姜黄素增加了INS-1细胞中葡萄糖转运蛋白2（GLUT2）的表达以及胰岛素受体（IR）、胰岛素受体底物-1（IRS1）、磷脂酰肌醇-3-激酶（PI3K）和AKT的磷酸化。此外，姜黄素刺激增加了PDX-1和GCK的表达。本研究表明，姜黄素通过激活INS-1细胞中的PI3K/Akt/GLUT2途径，防止高糖导致的胰岛素表达和分泌减少。
