Jilka R L
Research Service, Veterans Administration Medical Center, Kansas City, MO 64128.
Bone Miner. 1989 Jul;6(3):277-87. doi: 10.1016/0169-6009(89)90034-2.
Collagenolytic enzyme release from bone cells was studied using cultured calvarial cells which are capable of degrading calcified and noncalcified collagen (cells from normal mice) or only noncalcified collagen (cells from osteopetrotic (mi/mi) mice). Treatment of cells from either normal or mi/mi mice with parathyroid hormone (PTH) or lipopolysaccharide (LPS) resulted in the appearance of latent collagenolytic enzyme activity in the medium. Chromatography of media from cells from normal mice treated with PTH on lysine-Sepharose resulted in the separation of latent collagenase and latent gelatinase. Further characterization of the enzymes showed that they were similar to those previously isolated from media of calvaria cultured with heparin. Collagenase activity of media of cells from normal or mi/mi mice treated with PTH or LPS yielded identical elution patterns upon chromatography on lysine-Sepharose. These results show that the mi allele has no effect on regulation of latent collagenolytic enzyme release. The results show that the mi allele has no effect on regulation of latent collagenolytic enzyme release. The data also suggest that previously described differences between PTH- and LPS-stimulated collagen degradation in cultured calvaria are due to factors other than differences in the ability of these agents to stimulate the release of collagenolytic enzymes.
利用培养的颅骨细胞研究了骨细胞中胶原酶的释放情况，这些细胞能够降解钙化和未钙化的胶原蛋白（来自正常小鼠的细胞）或仅能降解未钙化的胶原蛋白（来自骨石化（mi/mi）小鼠的细胞）。用甲状旁腺激素（PTH）或脂多糖（LPS）处理正常或mi/mi小鼠的细胞后，培养基中出现了潜在的胶原酶活性。用赖氨酸-琼脂糖对经PTH处理的正常小鼠细胞的培养基进行层析，可分离出潜在的胶原酶和潜在的明胶酶。对这些酶的进一步表征表明，它们与先前从用肝素培养的颅骨培养基中分离出的酶相似。用赖氨酸-琼脂糖层析时，经PTH或LPS处理的正常或mi/mi小鼠细胞培养基的胶原酶活性产生相同的洗脱模式。这些结果表明，mi等位基因对潜在胶原酶释放的调节没有影响。结果表明，mi等位基因对潜在胶原酶释放的调节没有影响。数据还表明，先前描述的培养颅骨中PTH和LPS刺激的胶原降解之间的差异是由这些试剂刺激胶原酶释放能力差异以外的因素引起的。
