Stevenson D, Binggeli R, Weinstein R C, Keck J G, Lai M C, Tong M J
Liver Center, Huntington Memorial Hospital, Pasadena, California.
Cancer Res. 1989 Sep 1;49(17):4842-5.
One of the body's natural defense mechanisms against tumor cells is lysis of the invading cell by cytotoxic T-cells and natural killer (NK) cells. Five human hepatocellular carcinoma cell lines were found to have different sensitivities to killing by peripheral blood monocytes in a 51Cr release assay. This killing was demonstrated to be due to NK cell lysis. Electrical recording measurements of the membrane potentials of these five cell lines showed different values for each line, all below values reported for normal hepatocytes. Correlation between mean cell membrane potential, and sensitivity to NK lysis, revealed an inverse relationship. In this study we demonstrate that the lower the mean membrane potential of a human hepatocellular carcinoma cell line, the more sensitive it is to NK cell cytolysis. Cell surface positive potential did not correlate with NK cytolysis and only a weak correlation was found between cell membrane negative potential and cell surface positive potential between cell lines.
机体对抗肿瘤细胞的一种天然防御机制是细胞毒性T细胞和自然杀伤（NK）细胞对入侵细胞的裂解作用。在一项51Cr释放试验中，发现五种人肝癌细胞系对外周血单核细胞的杀伤具有不同的敏感性。这种杀伤作用被证明是由于NK细胞的裂解。对这五种细胞系的膜电位进行电记录测量，结果显示每个细胞系的值都不同，均低于正常肝细胞报道的值。平均细胞膜电位与对NK裂解的敏感性之间的相关性显示出一种负相关关系。在本研究中，我们证明人肝癌细胞系的平均膜电位越低，其对NK细胞胞溶作用就越敏感。细胞表面正电位与NK胞溶作用无关，并且在各细胞系之间，仅发现细胞膜负电位与细胞表面正电位之间存在微弱的相关性。
