Lai Xianyin, Liangpunsakul Suthat, Li Kaigang, Witzmann Frank A
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, IN, USA; Department of Cellular & Integrative Physiology, Indiana University School of Medicine, IN, USA.
Electrophoresis. 2015 Feb;36(4):556-63. doi: 10.1002/elps.201400319. Epub 2015 Jan 22.
Although numerous biomarkers or biomarker candidates have been discovered to detect levels of drinking and intervals of time after last drinking episode, only a few biomarkers have been applied to monitor abstinence in a longer interval (≥6 wks) from alcohol abuse. Considering sample sources, sensitivity, and specificity, new biomarkers from blood with better accuracy are needed. To address this, serum proteomic profiles were compared between pre- and post- treatment samples from subjects seeking treatment for alcohol abuse and dependence in an intensive 6 wk daily outpatient program using high-abundance plasma protein immunodepletion and LC-MS/MS techniques. Protein identification, quantification, candidate biomarker selection, and prioritization analyses were carried out. Among the 246 quantified serum proteins, abundance of 13 and 45 proteins in female and male subjects were significantly changed (p ≤ 0.05), respectively. Of these biomarker candidate proteins, 2 (female) and 8 (male) proteins were listed in category 1, with high area under the receiver operating characteristic curve, sensitivity, specificity, and fold change. In summary, several new biomarker candidates have been identified to monitor abstinence from alcohol abuse.
尽管已经发现了许多生物标志物或生物标志物候选物来检测饮酒量和上次饮酒事件后的时间间隔，但只有少数生物标志物被用于监测从酒精滥用开始较长时间间隔（≥6周）的戒酒情况。考虑到样本来源、敏感性和特异性，需要从血液中获取具有更高准确性的新生物标志物。为了解决这个问题，我们使用高丰度血浆蛋白免疫去除和液相色谱-串联质谱技术，比较了在一个为期6周的强化门诊治疗项目中寻求酒精滥用和依赖治疗的受试者治疗前后样本的血清蛋白质组图谱。进行了蛋白质鉴定、定量、候选生物标志物选择和优先级分析。在246种定量血清蛋白中，女性和男性受试者中分别有13种和45种蛋白的丰度发生了显著变化（p≤0.05）。在这些候选生物标志物蛋白中，有2种（女性）和8种（男性）蛋白被列为1类，具有较高的受试者工作特征曲线下面积、敏感性、特异性和倍数变化。总之，已经鉴定出了几种新的生物标志物候选物来监测酒精滥用的戒酒情况。
