Ahn Gee-Hyun, Moon Jin Seok, Shin So-Yeon, Min Won Ki, Han Nam Soo, Seo Jin-Ho
Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, South Korea.
J Ind Microbiol Biotechnol. 2015 Jan;42(1):49-55. doi: 10.1007/s10295-014-1553-x. Epub 2014 Dec 5.
The aim of this study was to develop a competitive quantitative-PCR (CQ-PCR) method for rapid analysis of the population dynamics of lactic acid bacteria (LAB) in kimchi. For this, whole chromosome sequences of Leuconostoc mesenteroides, Lactobacillus plantarum, and Lb. brevis were compared and species-specific PCR primers targeting dextransucrase, 16S rRNA, and surface layer protein D (SlpD) genes, respectively, were constructed. The tested strains were quantified both in medium and kimchi by CQ-PCR and the results were compared with the data obtained using a conventional plate-counting method. As a result, the three species were successfully detected and quantified by the indicated primer sets. Our results show that the CQ-PCR method targeting species-specific genes is suitable for rapid estimation of LAB population to be used in the food fermentation industry.
本研究的目的是开发一种竞争性定量聚合酶链反应（CQ-PCR）方法，用于快速分析泡菜中乳酸菌（LAB）的种群动态。为此，比较了肠系膜明串珠菌、植物乳杆菌和短乳杆菌的全染色体序列，并分别构建了靶向葡聚糖蔗糖酶、16S rRNA和表层蛋白D（SlpD）基因的种特异性PCR引物。通过CQ-PCR对测试菌株在培养基和泡菜中的数量进行了定量，并将结果与使用传统平板计数法获得的数据进行了比较。结果表明，所指示的引物组成功地检测和定量了这三个物种。我们的结果表明，靶向种特异性基因的CQ-PCR方法适用于食品发酵工业中乳酸菌种群的快速估计。
