Esmaeili Abolghasem, Pakravan Golnaz, Ghaedi Kamran, Noorbakhshnia Maryam
Cell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran -
Cell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
J Neurosurg Sci. 2018 Apr;62(2):146-152. doi: 10.23736/S0390-5616.16.03106-4. Epub 2014 Dec 5.
Neurotrophins as polypeptide growth factors have important roles during nervous system development and are involved in neuronal differentiation and survival and spinal cord reorganization. Neurotrophins have been recognized as factors which are involved in the development of damaged axons and increase the axon growth ability and neuroplasticity. Spinal cord injury (SCI) is associated with numerous physiological damages, leading to neuron death, axon extended destruction healthy and intact neurons demyelination, inflammation, cell death and severe motor/sensory defects. The aim of this study was to investigate the alteration in messenger RNA neurotrophin 4 and tyrosine kinase receptors B expression levels following SCI.
In this research, to know expression level alterations of neurotrophin 4 mRNA and its receptor Trk- B at 6 hours and 1, 3, 7 and 10 days after SCI, we developed competitive RT-qPCR. mRNA was extracted from T9 injury site (epicenter, rostral and caudal to the epicenter) and reversed to cDNA.
The results showed that the expression of these genes changed after SCI. The NT4 mRNA expression level in the rostral to the epicenter decreased after enhancement in the 1st 6 hours. At the epicenter and in the caudal to the epicenter, it decreased. mRNA expression level of Trk-B decreased after an increase in the initial hours in all the areas.
The present results showed that NT4 and Trk-B are expressed temporary and spatially following SCI and the adjustment of these neurotrophins rate in SCI may provide therapeutic benefits.
神经营养因子作为多肽生长因子，在神经系统发育过程中发挥重要作用，参与神经元分化、存活及脊髓重塑。神经营养因子已被公认为参与受损轴突发育、增强轴突生长能力和神经可塑性的因子。脊髓损伤（SCI）与多种生理损伤相关，导致神经元死亡、轴突广泛破坏、健康完整神经元脱髓鞘、炎症、细胞死亡及严重的运动/感觉缺陷。本研究旨在探讨脊髓损伤后信使核糖核酸神经营养因子4和酪氨酸激酶受体B表达水平的变化。
在本研究中，为了解脊髓损伤后6小时及1、3、7和10天神经营养因子4信使核糖核酸及其受体Trk-B的表达水平变化，我们开展了竞争性逆转录定量聚合酶链反应。从T9损伤部位（震中、震中头端和尾端）提取信使核糖核酸并逆转录为互补脱氧核糖核酸。
结果显示，脊髓损伤后这些基因的表达发生了变化。震中头端的神经营养因子4信使核糖核酸表达水平在最初6小时升高后下降。在震中及震中尾端，其表达水平下降。所有区域中，Trk-B信使核糖核酸表达水平在最初数小时升高后下降。
目前的结果表明，脊髓损伤后神经营养因子4和Trk-B在时间和空间上呈暂时性表达，调节脊髓损伤中这些神经营养因子的水平可能具有治疗益处。
