Cui Meiyu, Qiu Jinxue, Li Zhenghua, He Miao, Jin Mingshi, Kim Jiman, Quinto Maurizio, Li Donghao
Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Park Road 977, Yanji City, Jilin Province, China 133002.
Department of Chemistry, BK21 School of Chemical Materials Science, Department of Energy Science and SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, Korea.
Talanta. 2015 Jan;132:564-71. doi: 10.1016/j.talanta.2014.09.012. Epub 2014 Oct 7.
In this study, a stainless steel wire/ionic liquid-solid phase microextraction technique was developed for the direct extraction of APs from water samples. Some parameters were optimised, such as selection of the substrate and ILs, extraction time, extraction temperature, stirring rate and sample pH, etc. The experimental data demonstrated that the etched stainless steel wire was a suitable substrate for IL-coated SPME. The coating was prepared by directly depositing the ILs onto the surface of the etched stainless steel wire, which exhibited a porous structure and a high surface area. The [C8MIM][PF6] IL exhibited maximum efficiency with an extraction time of 30 min, and the aqueous sample was maintained at 40 °C and adjusted to pH 2 under stirring conditions. The enrichment factor of the IL coating for the four APs ranged from 1382 to 4779, the detection limits (LOD, S/N=3) of the four APs ranged from 0.01 to 0.04 ng mL(-1) and the RSD values for purified water spiked with APs ranged from 4.0 to 11.8% (n=3). The calibration graphs were linear in the concentration range from 0.5 to 200 ng mL(-1) (R(2)>0.9569). The optimised method was successfully applied for the analysis of real water samples, and the method was suitable for the extraction of APs from water samples.
在本研究中，开发了一种不锈钢丝/离子液体-固相微萃取技术，用于直接从水样中萃取抗生素。对一些参数进行了优化，如底物和离子液体的选择、萃取时间、萃取温度、搅拌速率和样品pH值等。实验数据表明，蚀刻不锈钢丝是用于离子液体涂层固相微萃取的合适底物。通过将离子液体直接沉积在蚀刻不锈钢丝表面制备涂层，该涂层呈现多孔结构和高表面积。[C8MIM][PF6]离子液体在萃取时间为30分钟时表现出最大效率，水样在搅拌条件下保持在40℃并调节至pH 2。离子液体涂层对四种抗生素的富集因子范围为1382至4779，四种抗生素的检测限（LOD，S/N = 3）范围为0.01至0.04 ng mL(-1)，加标纯化水的相对标准偏差（RSD）值范围为4.0至11.8%（n = 3）。校准曲线在0.5至200 ng mL(-1)的浓度范围内呈线性（R(2)>0.9569）。该优化方法成功应用于实际水样的分析，该方法适用于从水样中萃取抗生素。
