Indiveri C, Palmieri F
Dipartimento Farmaco-iologico, Università di Bari, Italy.
FEBS Lett. 1989 Aug 14;253(1-2):217-20. doi: 10.1016/0014-5793(89)80962-7.
The carnitine carrier from rat liver mitochondria has been extracted with Triton X-100 ad partially purified by chromatography on hydroxyapatite and celite. During purification the activity of the carrier was monitored by functional reconstitution into liposomes. The purified fraction is 250-fold enriched with respect to the N-ethylmaleimide-sensitive carnitine/carnitine transport activity. The substrate specificity and the inhibitor sensitivity of carnitine transport in liposomes resemble closely those described for the transport of carnitine in mitochondria.
已用 Triton X-100 从大鼠肝线粒体中提取肉碱载体，并通过在羟磷灰石和硅藻土上进行色谱法进行部分纯化。在纯化过程中，通过功能重建到脂质体中来监测载体的活性。纯化后的组分相对于对 N-乙基马来酰亚胺敏感的肉碱/肉碱转运活性富集了 250 倍。脂质体中肉碱转运的底物特异性和抑制剂敏感性与线粒体中肉碱转运的描述非常相似。
