2,3-丁二酮对人髓过氧化物酶的影响。

Effect of 2,3-butanedione on human myeloperoxidase.

Willassen N P, Little C

Institute of Medical Biology, University of Tromsø, Norway.

Int J Biochem. 1989;21(7):755-9. doi: 10.1016/0020-711x(89)90207-3.

Abstract

Myeloperoxidase is inhibited by various diketones that are recognized arginine reagents. 2. Although arginine residues in the enzyme were modified in both the light and the dark, enzyme inactivation occurred only in the presence of light. 3. Under conditions where diketones caused inactivation of myeloperoxidase, spectral studies indicated marked damage to the haem residues of the enzyme. 4. It was concluded that diketones serve simply as photosensitizers of visible light-induced inactivation of myeloperoxidase. 5. Studies on other haemoproteins indicated the great ease with which the presence of diketones sensitized haem residues for photodestruction.

摘要

髓过氧化物酶被各种作为精氨酸试剂的二酮所抑制。2. 尽管酶中的精氨酸残基在光照和黑暗条件下均被修饰，但酶失活仅在光照条件下发生。3. 在二酮导致髓过氧化物酶失活的条件下，光谱研究表明该酶的血红素残基受到显著损伤。4. 得出的结论是，二酮仅作为可见光诱导的髓过氧化物酶失活的光敏剂。5. 对其他血红素蛋白的研究表明，二酮的存在很容易使血红素残基对光破坏敏感。