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RAB8A是子宫内膜癌的一种新生物标志物？

RAB8A a new biomarker for endometrial cancer?

作者信息

Bie Yachun, Zhang Zhenyu

机构信息

Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China.

出版信息

World J Surg Oncol. 2014 Dec 4;12:371. doi: 10.1186/1477-7819-12-371.

DOI:10.1186/1477-7819-12-371
PMID:25477298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4289371/
Abstract

BACKGROUND

We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).

METHODS

Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.

RESULTS

The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.

CONCLUSIONS

RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.

摘要

背景

我们旨在找出树突状细胞（DCs）与肿瘤抗原脉冲DCs之间的差异蛋白质，以帮助寻找子宫内膜癌（EC）的新型生物标志物。

方法

从脐带血中分离出单核细胞，并用细胞因子将其诱导为DCs。通过液相色谱串联质谱法（LC-MS/MS）对DCs和肿瘤裂解物脉冲DCs进行比较蛋白质组学分析。通过蛋白质免疫印迹分析鉴定差异蛋白质。

结果

发现Ras相关蛋白Rab-8A（RAB8A）在两种细胞中的表达存在差异。这种现象在子宫内膜癌组织中也得到了证实。

结论

RAB8A可能是子宫内膜癌的新型生物标志物。利用LC-MS/MS对DCs和肿瘤裂解物脉冲DCs进行比较蛋白质组学分析可能有助于我们找到癌症新的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/445d4ec3b80f/12957_2014_1867_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/a36611c556a0/12957_2014_1867_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/980c965fef9b/12957_2014_1867_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/2172e9513a51/12957_2014_1867_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/445d4ec3b80f/12957_2014_1867_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/a36611c556a0/12957_2014_1867_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/980c965fef9b/12957_2014_1867_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/2172e9513a51/12957_2014_1867_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c6/4289371/445d4ec3b80f/12957_2014_1867_Fig4_HTML.jpg

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