东北印度主要疟疾传播媒介——微小按蚊线粒体区域的遗传多样性和基因结构。

Genetic diversity and gene structure of mitochondrial region of Anopheles minimus (Diptera: Culicidae) - major malaria vector of North east India.

机构信息

Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.

出版信息

Asian Pac J Trop Med. 2014 Dec;7(12):952-5. doi: 10.1016/S1995-7645(14)60168-1.

DOI:10.1016/S1995-7645(14)60168-1

PMID:25479623

Abstract

OBJECTIVE

To depict mitochondrial genetic variation for the first time among Anopheles minimus (An.minimus) (Diptera: Culicidae) species from two malaria endemic states of NE India.

METHODS

Phylogeographic analysis was carried at 9 out of 12 sites of An.minimus confirmed malaria endemic places.

RESULTS

All sequences were Adenine-Thymine rich regions. Transitions were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 case non synonymous mutation was observed. Three distinct clusters of haplotypes were generated. Haplotype diversity and low nucleotide diversity were studied. Overall negative values obtained from Tajima's D test and Fu'sFS test indicate a recent genetic population expansion. Network analysis has explained sequence diversity that was also shown by mutations in 6 sequences.

CONCLUSIONS

High genetic diversity observed within the populations of An.minimus species has several possible implications for vector control in the region.

摘要

目的

首次描述来自印度东北部两个疟疾流行州的微小按蚊（An.minimus）（双翅目：蚊科）种的线粒体遗传变异。

方法

在确认疟疾流行的 12 个地点中的 9 个地点进行了系统地理分析。

结果

所有序列均富含腺嘌呤-胸腺嘧啶。在 6 个序列中观察到转换，其中 5 个突变是同义替换，在 1 个情况下观察到非同义突变。生成了三个不同的单倍型簇。研究了单倍型多样性和低核苷酸多样性。Tajima 的 D 检验和 Fu 的 FS 检验得出的总体负值表明最近的遗传种群扩张。网络分析解释了 6 个序列中的突变所显示的序列多样性。