Dutta P, Khan S A, Topno R, Chowdhury P, Baishya M, Prakash A, Bhattacharyya D R, Mahanta J
Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
Trop Biomed. 2013 Sep;30(3):552-8.
Anopheles minimus is one of the major vectors for transmission of malaria disease in north eastern (NE) region of India. The minimus species complex of Minimus subgroup of Myzomyia series of anophelines were studied in malaria affected states--Assam and Arunachal Pradesh (AP) of NE India. Ribosomal DNA markers--second internal transcribed spacer (ITS2) and third domain (D3) of 28S gene were used to characterize An. minimus species. Sequence homogeneity was observed in D3 sequences of An.minimus specimens throughout both the states. However, a transversion in ITS2 sequence of single specimen collected from Assam-Meghalaya border areas illustrates possibility of intra population polymorphism in ITS2 sequence within the geographical region.
微小按蚊是印度东北部地区疟疾传播的主要媒介之一。在印度东北部受疟疾影响的阿萨姆邦和阿鲁纳恰尔邦，对按蚊亚属Myzomyia系列的微小按蚊亚组的微小按蚊物种复合体进行了研究。核糖体DNA标记——28S基因的第二内部转录间隔区（ITS2）和第三结构域（D3）被用于鉴定微小按蚊物种。在两个邦的微小按蚊标本的D3序列中均观察到序列同源性。然而，从阿萨姆邦-梅加拉亚邦边境地区采集的单个标本的ITS2序列中出现了一个颠换，这表明该地理区域内ITS2序列存在种群内多态性的可能性。
