Corapi W V, French T W, Dubovi E J
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14851.
J Virol. 1989 Sep;63(9):3934-43. doi: 10.1128/JVI.63.9.3934-3943.1989.
Seven calves between 1 week and 2 months of age were infected with a noncytopathic field isolate of bovine viral diarrhea virus (BDV) in order to evaluate the effect of BDV infection on the concentration of circulating platelets in the blood. All calves were determined to be free of BDV and neutralizing antibodies to BDV before infection. Platelet counts were performed on a daily basis over a 30-day period beginning at the time of infection. By 2 weeks postinfection, all calves showed a significant drop in the number of circulating platelets and a marked hyperplasia of megakaryocytes in the bone marrow. In three of the seven calves, thrombocytopenia was severe (less than or equal to 5,000/microliters) for 1 to 6 days. In two of these three animals, extensive petechial and ecchymotic hemorrhages were observed on all mucosal surfaces and on various internal organs during the period of severe thrombocytopenia. BDV was consistently isolated from the platelets during the early phases of the infection, and viral antigen was occasionally detected on platelets by a fluorescent-antibody assay. The results demonstrate that BDV infection is associated with decreases in platelet numbers and suggest that platelets may serve as carriers of circulating virus.
为了评估牛病毒性腹泻病毒（BDV）感染对血液中循环血小板浓度的影响，对7头1周龄至2月龄的犊牛感染了一种非细胞病变的BDV野毒株。所有犊牛在感染前均被确定未感染BDV且不存在针对BDV的中和抗体。从感染时开始，在30天的时间内每天进行血小板计数。感染后2周时，所有犊牛的循环血小板数量均显著下降，且骨髓中巨核细胞明显增生。7头犊牛中有3头严重血小板减少（小于或等于5000/微升）持续1至6天。在这3头动物中的2头，在严重血小板减少期间，在所有粘膜表面和各种内脏器官上均观察到广泛的瘀点和瘀斑出血。在感染早期，始终能从血小板中分离出BDV，并且偶尔通过荧光抗体试验在血小板上检测到病毒抗原。结果表明，BDV感染与血小板数量减少有关，并提示血小板可能作为循环病毒的载体。
