中和抗体GNbAC1可消除人内源性逆转录病毒-W包膜蛋白介导的少突胶质细胞成熟阻滞。
The neutralizing antibody GNbAC1 abrogates HERV-W envelope protein-mediated oligodendroglial maturation blockade.
作者信息
Kremer David, Förster Moritz, Schichel Tanja, Göttle Peter, Hartung Hans-Peter, Perron Hervé, Küry Patrick
机构信息
Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany/These authors contributed equally to this study.
Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.
出版信息
Mult Scler. 2015 Aug;21(9):1200-3. doi: 10.1177/1352458514560926. Epub 2014 Dec 5.
BACKGROUND
The envelope protein (ENV) of the human endogenous retrovirus type W is implicated in inflammatory reactions in multiple sclerosis (MS) but also interferes with oligodendroglial maturation. A neutralizing antibody GNbAC1 has been developed and successfully been tested in clinical trials.
OBJECTIVES AND METHODS
We stimulated primary oligodendroglial cells with ENV upon preincubation with GNbAC1 and assessed for nitrosative stress and myelin expression.
RESULTS
Neutralization of ENV by GNbAC1 reduces its ability to induce stress reactions resulting in a rescue of myelin expression.
CONCLUSIONS
Beyond immune cell modulation, this monoclonal antibody may therefore help to overcome the oligodendroglial differentiation blockade in MS.
背景
人类内源性W型逆转录病毒的包膜蛋白（ENV）与多发性硬化症（MS）的炎症反应有关，但也会干扰少突胶质细胞的成熟。一种中和抗体GNbAC1已被研发出来，并在临床试验中成功进行了测试。
目的和方法
我们在GNbAC1预孵育后用ENV刺激原代少突胶质细胞，并评估亚硝化应激和髓鞘表达情况。
结果
GNbAC1对ENV的中和作用降低了其诱导应激反应的能力，从而挽救了髓鞘表达。
结论
因此，除了免疫细胞调节作用外，这种单克隆抗体可能有助于克服MS中少突胶质细胞分化阻滞的问题。
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