微小RNA-206通过靶向囊泡相关膜蛋白2来调节表面活性剂的分泌。
MicroRNA-206 regulates surfactant secretion by targeting VAMP-2.
作者信息
Zhang Honghao, Guo Yujie, Mishra Amarjit, Gou Deming, Chintagari Narendranath Reddy, Liu Lin
机构信息
Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States.
Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States; Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK 73034, United States.
出版信息
FEBS Lett. 2015 Jan 2;589(1):172-6. doi: 10.1016/j.febslet.2014.11.043. Epub 2014 Dec 4.
Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UTR) luciferase assay. Mutations in the predicated miR-206 binding sites reduced the binding of miR-206 to the 3'-UTR of VAMP-2. miR-206 decreased the expression of VAMP-2 protein and decreased the lung surfactant secretion in alveolar type II cells. In conclusion, miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein.
肺表面活性物质的分泌是一个高度受调控的过程。我们之前的研究表明，VAMP - 2对表面活性物质的分泌至关重要。在本研究中，我们通过VAMP - 2研究了miR - 206在表面活性物质分泌中的作用。通过3' - 非翻译区（3' - UTR）荧光素酶测定证实VAMP - 2是miR - 206的靶标。预测的miR - 206结合位点的突变减少了miR - 206与VAMP - 2的3' - UTR的结合。miR - 2六降低了VAMP - 2蛋白的表达，并减少了II型肺泡细胞中肺表面活性物质的分泌。总之，miR - 206通过限制VAMP - 2蛋白的可用性来调节肺表面活性物质的分泌。
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