鉴定在病原体相关分子模式（PAMP）处理后被激活的其他丝裂原活化蛋白激酶（MAP激酶）。
Identification of additional MAP kinases activated upon PAMP treatment.
作者信息
Nitta Yukino, Ding Pingtao, Zhang Yuelin
机构信息
a Department of Botany ; University of British Columbia ; Vancouver , BC Canada.
出版信息
Plant Signal Behav. 2014;9(11):e976155. doi: 10.4161/15592324.2014.976155.
Abstract
Mitogen-activated protein (MAP) kinase cascades play important roles in plant immunity. Upon pathogen associated molecular pattern (PAMP) treatment, MPK3, MPK6 and MPK4 are quickly activated by upstream MKKs through phosphorylation. Western blot analysis using α-phospho-p44/42-ERK antibody suggests that additional MPKs with similar size as MPK4 are also activated upon PAMP perception. To identify these MAP kinases, 7 candidate MPKs with similar sizes as MPK4 were selected for further analysis. Transgenic plants expressing these MPKs with a ZZ-3xFLAG double tag of 17 kD were generated and analyzed by western blot. MPK1, MPK11 and MPK13 were found to be phosphorylated upon treatment with flg22. Our study revealed additional MAPKs being activated during PAMP-triggered immunity.
摘要
丝裂原活化蛋白（MAP）激酶级联在植物免疫中发挥重要作用。在病原体相关分子模式（PAMP）处理后，MPK3、MPK6和MPK4通过上游MKKs的磷酸化迅速被激活。使用α-磷酸化-p44/42-ERK抗体的蛋白质免疫印迹分析表明，在感知PAMP时，与MPK4大小相似的其他MPK也被激活。为了鉴定这些MAP激酶，选择了7个与MPK4大小相似的候选MPK进行进一步分析。生成了表达带有17 kD的ZZ-3xFLAG双标签的这些MPK的转基因植物，并通过蛋白质免疫印迹进行分析。发现用flg22处理后MPK1、MPK11和MPK13被磷酸化。我们的研究揭示了在PAMP触发的免疫过程中还有其他MAPK被激活。
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Annu Rev Phytopathol. 2013;51:245-66. doi: 10.1146/annurev-phyto-082712-102314. Epub 2013 May 6.
2
Phosphorylation of an ERF transcription factor by Arabidopsis MPK3/MPK6 regulates plant defense gene induction and fungal resistance.
Plant Cell. 2013 Mar;25(3):1126-42. doi: 10.1105/tpc.112.109074. Epub 2013 Mar 22.
3
Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases.
Plant Physiol. 2013 Apr;161(4):2146-58. doi: 10.1104/pp.112.212431. Epub 2013 Feb 19.
4
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Cell Host Microbe. 2012 Mar 15;11(3):253-63. doi: 10.1016/j.chom.2012.01.015.
5
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Mol Plant Microbe Interact. 2012 Apr;25(4):471-80. doi: 10.1094/MPMI-11-11-0281.
6
A major role of the MEKK1-MKK1/2-MPK4 pathway in ROS signalling.
Mol Plant. 2009 Jan;2(1):120-37. doi: 10.1093/mp/ssn079. Epub 2009 Jan 6.
7
Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling.
Proc Natl Acad Sci U S A. 2009 May 12;106(19):8067-72. doi: 10.1073/pnas.0810206106. Epub 2009 Apr 29.
8
MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plants.
Cell Res. 2008 Dec;18(12):1190-8. doi: 10.1038/cr.2008.300.
9
Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1.
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MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.
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