Wang Zheng, Bao Ling-Li, Zhao Feng-Yun, Tang Min-Qiang, Chen Ting, Li Yaoming, Wang Bing-Xu, Fu Benzhong, Fang Hedi, Li Guan-Ying, Cao Jun, Ding Li-Na, Zhu Ke-Ming, Liu Sheng-Yi, Tan Xiao-Li
Institute of Life Sciences, Jiangsu University, Zhenjiang, China.
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.
Front Plant Sci. 2019 Feb 8;10:91. doi: 10.3389/fpls.2019.00091. eCollection 2019.
The disease caused by has traditionally been difficult to control, resulting in tremendous economic losses in oilseed rape (). Identification of important genes in the defense responses is critical for molecular breeding, an important strategy for controlling the disease. Here, we report that a mitogen-activated protein kinase gene, , plays an important role in the defense against in oilseed rape. is highly expressed in the stems, flowers and leaves, and its product is localized in the nucleus. Furthermore, is highly responsive to infection by and treatment with jasmonic acid (JA) or the biosynthesis precursor of ethylene (ET), but not to treatment with salicylic acid (SA) or abscisic acid. Moreover, overexpression (OE) of in and results in significantly enhanced resistance to , whereas resistance is diminished in RNAi transgenic plants. After infection, defense responses associated with ET, JA, and SA signaling are intensified in the -OE plants but weakened in the -RNAi plants when compared to those in the wild type plants; by contrast the level of both HO accumulation and cell death exhibits a reverse pattern. The candidate gene association analyses show that the BnaMPK3-encoding locus is a cause of variation in the resistance to in natural population. These results suggest that is a key regulator of multiple defense responses to , which may guide the resistance improvement of oilseed rape and related economic crops.
由[病原体名称未给出]引起的病害传统上难以控制，导致油菜（[油菜品种未给出]）遭受巨大经济损失。鉴定防御反应中的重要基因对于分子育种至关重要，而分子育种是控制该病害的一项重要策略。在此，我们报道一个丝裂原活化蛋白激酶基因[基因名称未给出]在油菜对[病原体名称未给出]的防御中起重要作用。[基因名称未给出]在茎、花和叶中高度表达，其产物定位于细胞核。此外，[基因名称未给出]对[病原体名称未给出]感染以及茉莉酸（JA）或乙烯（ET）生物合成前体处理高度响应，但对水杨酸（SA）或脱落酸处理不响应。而且，在[植物品种未给出]和[植物品种未给出]中过表达（OE）[基因名称未给出]会导致对[病原体名称未给出]的抗性显著增强，而在RNAi转基因植物中抗性减弱。在[病原体名称未给出]感染后，与ET、JA和SA信号相关的防御反应在[基因名称未给出]-OE植物中增强，但在[基因名称未给出]-RNAi植物中相对于野生型植物减弱；相比之下，HO积累和细胞死亡水平呈现相反模式。候选基因关联分析表明，编码[基因名称未给出]的BnaMPK3位点是自然[油菜品种未给出]群体中对[病原体名称未给出]抗性变异的一个原因。这些结果表明，[基因名称未给出]是对[病原体名称未给出]多种防御反应的关键调节因子，这可能为油菜及相关经济作物的抗性改良提供指导。
