Liang Rishen, Wang Chao, Zou Qing, Zhou Aiguo, Zhou Meng
a College of Life Science, Zhongkai University of Agriculture and Engineering , Guangzhou , People's Republic of China .
b Qingyuan Animal Husbandry and Fishery Technology Extension Station , Guangzhou , People's Republic of China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):2209-14. doi: 10.3109/19401736.2014.982628. Epub 2014 Dec 8.
Molecular phylogenetic topologies from 40 individuals of 17 sweetlips were constructed based on the mitochondrial cytochrome b (cyt b) and cytochrome c oxidase subunit I (COI) genes. All phylogenetic results strongly revealed the division of the sweetlips into three morphological distinct groups. Group I: sweetlips with colorful patterns, Group II and Group III: species with uniformly dark patterns. The relationships of those morphologically confused Plectorhinchus species were well-resolved in the phylogenetic results. We also confirmed that the genus Diagramma was placed inside the colorful Plectorhinchus groups, suggesting close relationship of Diagramma within Plectorhinchus. Additionally, we found that two species, P. orientalis and P. vittatus, which were traditionally considered as synonyms for oriental sweetlips, which were suggested to be two distinct species. Sequence divergence also revealed a great genetic variation between P. orientalis and P. vittatus (6.0% in Cyt b and 7.4% in COI), which was largely greater than the species diagnosis divergence value (2%) suggested by Hebert et al. This new finding suggested P. orientalis and P. vittatus might be two distinct species and should not be placed as synonyms.
基于线粒体细胞色素b（cyt b）和细胞色素c氧化酶亚基I（COI）基因，构建了17种姬鲷属鱼类40个个体的分子系统发育拓扑结构。所有系统发育结果都有力地表明，姬鲷属鱼类分为三个形态上明显不同的类群。第一类群：具有彩色斑纹的姬鲷；第二类群和第三类群：具有均匀深色斑纹的物种。在系统发育结果中，那些形态上容易混淆的姬鲷属物种之间的关系得到了很好的解析。我们还证实，双带姬鲷属被置于具有彩色斑纹的姬鲷类群中，这表明双带姬鲷属与姬鲷属关系密切。此外，我们发现，传统上被认为是东方姬鲷同义词的东方姬鲷和条纹姬鲷，被认为是两个不同的物种。序列差异还揭示了东方姬鲷和条纹姬鲷之间存在很大的遗传变异（细胞色素b中为6.0%，COI中为7.4%），这大大高于赫伯特等人提出的物种诊断差异值（2%）。这一新发现表明，东方姬鲷和条纹姬鲷可能是两个不同的物种，不应被视为同义词。
