Stringfellow D A, Lauerman L H, Thomson M S
Department of Pathobiology, College of Veterinary Medicine and Auburn University, AL 36849.
Am J Vet Res. 1989 Jun;50(6):990-2.
Preimplantation bovine ova were exposed in vitro to vesicular stomatitis virus, Indiana serotype, to document adherence of the virus to the zona pellucida. To determine the efficacy of this treatment, some of the ova were treated with trypsin after exposure to the virus. Vesicular stomatitis virus was isolated from 5 of 10 groups of zona pellucida-intact ova after 12 sequential washes without trypsin treatment. Vesicular stomatitis virus was also isolated from 4 of 11 groups of zona pellucida-intact ova after trypsin treatment.
