González-Garza M T, Said-Fernández S
Arch Invest Med (Mex). 1989 Jan-Mar;20(1):29-32.
Monophasic liquid media for axenical cultivation of Entamoeba histolytica, have permitted to define diverse aspects of this parasite's biology, without associated organisms interference. In addition, these media have allowed the in vitro evaluation of new drugs with potential antiamebic properties. PEHPS, a new medium recently developed in our laboratory, was used in order to determine its usefulness in the searching for new antiamebic compounds. The antiamebic potency of currently employed drugs in clinical therapy: emetine, metronidazole and diiodohydroxyquinoline on E. histolytica, strain HM-1, trophozoites grown in PEHPS was tested. The IC50 for each drug was 0.082 microgram/ml, 0.10 micrograms/ml and 5.84 micrograms/ml respectively. Results obtained with each drug are statistically reliable and reproducible, and agree with the species sensibility reported by several other authors. Accordingly, we propose PEHPS as a useful medium for new antiamebic drug research.
用于溶组织内阿米巴无菌培养的单相液体培养基，已能够在无相关生物干扰的情况下明确该寄生虫生物学的不同方面。此外，这些培养基还允许对具有潜在抗阿米巴特性的新药进行体外评估。PEHPS是我们实验室最近开发的一种新培养基，用于确定其在寻找新的抗阿米巴化合物方面的效用。测试了目前临床治疗中使用的药物：吐根碱、甲硝唑和二碘羟基喹啉对在PEHPS中生长的溶组织内阿米巴HM-1株滋养体的抗阿米巴效力。每种药物的IC50分别为0.082微克/毫升、0.10微克/毫升和5.84微克/毫升。每种药物获得的结果在统计学上是可靠且可重复的，并且与其他几位作者报道的该物种敏感性一致。因此，我们建议将PEHPS作为一种用于新抗阿米巴药物研究的有用培养基。
