Division of Newborn Medicine, Floating Hospital for Children at Tufts Medical Center , Boston, MA , USA.
Tufts Medical Center, The Institute for Clinical Research and Health Policy Studies , Boston, MA , USA ; Tufts Clinical and Translational Science Institute, Tufts University , Boston, MA , USA.
Front Pediatr. 2014 Nov 21;2:131. doi: 10.3389/fped.2014.00131. eCollection 2014.
We aimed to detect C-reactive protein (CRP) in neonatal saliva and evaluate its diagnostic utility.
Salivary and serum samples (n = 89) were collected from 40 neonates. Salivary CRP levels were determined using an enzyme-linked immunosorbent assay; serum CRP was measured per hospital protocol. Correlation coefficients with 95% confidence intervals and robust linear regression measured association while receiver-operator characteristic curves described the accuracy of salivary CRP in discriminating abnormal serum CRP thresholds of ≥10 and 5 mg/L. Corresponding sensitivities and specificities were calculated for these salivary cutpoints.
The area under the curve for salivary CRP in predicting serum CRP levels of ≥10 and 5 mg/L were 0.81 and 0.76, respectively. The corresponding sensitivity and specificity for raw salivary CRP to discriminate a serum CRP of ≥5 mg/L was 0.54 and 0.95, respectively. The corresponding sensitivity and specificity for raw salivary CRP to discriminate a serum CRP of ≥10 mg/L was 0.64 and 0.94, respectively. A statistically significant correlation was observed between serum and salivary CRP (r = 0.62, p < 0.001).
C-reactive protein is detectable in neonatal saliva and can predict abnormal serum CRP thresholds. Salivary CRP analysis represents a feasible screening tool for detecting abnormal serum CRP levels.
我们旨在检测新生儿唾液中的 C 反应蛋白（CRP），并评估其诊断效用。
收集 40 名新生儿的唾液和血清样本（n=89）。使用酶联免疫吸附测定法测定唾液 CRP 水平；根据医院方案测定血清 CRP。用 95%置信区间和稳健线性回归分析相关系数，以描述唾液 CRP 区分异常血清 CRP 阈值≥10 和 5mg/L 的准确性。计算这些唾液切点的相应灵敏度和特异性。
唾液 CRP 预测血清 CRP 水平≥10 和 5mg/L 的曲线下面积分别为 0.81 和 0.76。原始唾液 CRP 区分血清 CRP≥5mg/L 的灵敏度和特异性分别为 0.54 和 0.95。原始唾液 CRP 区分血清 CRP≥10mg/L 的灵敏度和特异性分别为 0.64 和 0.94。血清和唾液 CRP 之间存在统计学显著相关性（r=0.62，p<0.001）。
CRP 可在新生儿唾液中检测到，可预测异常血清 CRP 阈值。唾液 CRP 分析代表一种可行的筛查工具，用于检测异常的血清 CRP 水平。
