Miller L P, Perry D C
Neuroscience Lab, Veteran's Administration Medical Center, Washington, DC 20422.
Brain Res. 1989 Aug 28;495(2):367-72. doi: 10.1016/0006-8993(89)90230-8.
A role for endogenous opioids in trauma-induced brain injury has been supported by pharmacological studies. The present series of experiments were initiated to extend these observations by measuring opiate receptor subtype binding in gerbil hippocampus following 7 days recovery from a 10 min ischemic insult. Quantitative in vitro autoradiography was utilized to measure mu [( 3H]DAGO), kappa [( 3H]bremazocine + 10 microM morphiceptin + 100 nM DSLET), delta [( 3H]DSLET + 10 microM morphiceptin) and lambda [( 3H]naloxone + 300 nM diprenorphine) binding. While ischemic tissue samples at the level of the dorsal hippocampus showed complete loss of CA1 pyramidal cells, we observed no significant alterations in mu or delta binding suggesting a non-pyramidal cell localization of these receptors. Kappa binding decreased significantly to 88% of control in the CA1 and CA3 regions while lambda binding in the stratum lucidum (CA3) increased to 165% of control. Our results show that opiate receptor subtypes are differentially affected by an ischemic insult.
药理学研究支持内源性阿片类物质在创伤性脑损伤中发挥作用。本系列实验旨在通过测量沙土鼠海马在经历10分钟缺血性损伤并恢复7天后的阿片受体亚型结合情况，来扩展这些观察结果。采用定量体外放射自显影法测量μ[（3H）DAGO]、κ[（3H）布瑞马佐辛+10微摩尔吗啡肽+100纳摩尔DSLET]、δ[（3H）DSLET+10微摩尔吗啡肽]和λ[（3H）纳洛酮+300纳摩尔二丙诺啡]的结合情况。虽然背侧海马水平的缺血组织样本显示CA1锥体细胞完全丧失，但我们观察到μ或δ结合无显著变化，提示这些受体定位于非锥体细胞。κ结合在CA1和CA3区域显著下降至对照的88%，而透明层（CA3）中的λ结合增加至对照的165%。我们的结果表明，阿片受体亚型受缺血性损伤的影响存在差异。
