Demina E P, Miroshnikova V V, Maĭorov N V, Davydenko V V, Shvartsman A L
Tsitologiia. 2013;55(8):580-5.
ABCA1 transporter is one of the key factors defining the level of antiatherogenic HDL in plasma. It is actively involved in the removal of cholesterol from peripheral tissues by reverse cholesterol transport. However, the influence of the level of ABCA1 mRNA and the level of ABCA1 protein 1 in macrophages in atherosclerosis remains unexplored. Using real time PCR we determined ABCA1 mRNA level in macrophages cultured for 5 days with macrophage colony-stimulating factor (M-CSF). ABCA1 mRNA levels in macrophages from patients with arterial stenosis were increased when compared with the control group, P = 0.04. According to a Western blot analysis ABCA1 protein level in macrophages from patients was significantly lower than in the control group, P = 0.01. Our results suggest that the level of ABCA1 mRNA and level of ABCA1 protein in macrophages may be important factors in the development of atherosclerosis.
ABCA1转运蛋白是决定血浆中抗动脉粥样硬化高密度脂蛋白水平的关键因素之一。它通过逆向胆固醇转运积极参与外周组织中胆固醇的清除。然而，ABCA1信使核糖核酸水平和动脉粥样硬化中巨噬细胞内ABCA1蛋白1水平的影响仍未得到研究。我们使用实时聚合酶链反应测定了用巨噬细胞集落刺激因子（M-CSF）培养5天的巨噬细胞中的ABCA1信使核糖核酸水平。与对照组相比，动脉狭窄患者巨噬细胞中的ABCA1信使核糖核酸水平升高，P = 0.04。根据蛋白质免疫印迹分析，患者巨噬细胞中的ABCA1蛋白水平显著低于对照组，P = 0.01。我们的结果表明，巨噬细胞中ABCA1信使核糖核酸水平和ABCA1蛋白水平可能是动脉粥样硬化发展的重要因素。
