一种快速、简单且廉价的制备链特异性RNA测序文库的方法。

A rapid, simple, and inexpensive method for the preparation of strand-specific RNA-Seq libraries.

作者信息

Hunt Arthur G

机构信息

Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA,

出版信息

Methods Mol Biol. 2015;1255:195-207. doi: 10.1007/978-1-4939-2175-1_17.

DOI:10.1007/978-1-4939-2175-1_17

PMID:25487215

Abstract

High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.

摘要

短cDNA标签的高通量测序，即RNA测序，已成为植物全基因组基因表达研究的主要手段。RNA测序文库必然包含与mRNA - 聚腺苷酸（poly(A)）连接点或聚腺苷酸化位点相对应的标签，因此可以挖掘这些数据以帮助研究可变聚腺苷酸化。本报告介绍了一种从不同量的总RNA制备链特异性RNA测序文库的简单、快速且廉价的方法。