Department of Crop Science, North Carolina State University, Raleigh, NC, USA.
Pastoral Genomics, c/o ViaLactia Biosciences (NZ) Ltd/Fonterra, Auckland, New Zealand.
Plant Biotechnol J. 2015 Jun;13(5):689-99. doi: 10.1111/pbi.12291. Epub 2014 Dec 9.
HUB1, also known as Ubl5, is a member of the subfamily of ubiquitin-like post-translational modifiers. HUB1 exerts its role by conjugating with protein targets. The function of this protein has not been studied in plants. A HUB1 gene, LpHUB1, was identified from serial analysis of gene expression data and cloned from perennial ryegrass. The expression of this gene was reported previously to be elevated in pastures during the summer and by drought stress in climate-controlled growth chambers. Here, pasture-type and turf-type transgenic perennial ryegrass plants overexpressing LpHUB1 showed improved drought tolerance, as evidenced by improved turf quality, maintenance of turgor and increased growth. Additional analyses revealed that the transgenic plants generally displayed higher relative water content, leaf water potential, and chlorophyll content and increased photosynthetic rate when subjected to drought stress. These results suggest HUB1 may play an important role in the tolerance of perennial ryegrass to abiotic stresses.
HUB1，也称为 Ubl5，是泛素样蛋白翻译后修饰亚家族的成员。HUB1 通过与蛋白质靶标缀合发挥其作用。该蛋白在植物中的功能尚未研究过。从基因表达序列分析数据中鉴定出一个 HUB1 基因，LpHUB1，并从多年生黑麦草中克隆得到。先前的报道称，该基因在夏季牧场和气候控制生长室中的干旱胁迫下表达上调。在这里，过表达 LpHUB1 的牧场型和草坪型转基因多年生黑麦草植物表现出增强的耐旱性，表现在草坪质量提高、膨压维持和生长增加。进一步的分析表明，在受到干旱胁迫时，转基因植物通常表现出更高的相对含水量、叶片水势和叶绿素含量以及增加的光合速率。这些结果表明 HUB1 可能在多年生黑麦草对非生物胁迫的耐受中发挥重要作用。
