• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CYC1 UAS1中的一个点突变产生了一种新的调控元件组合，可协同激活转录。

A point mutation in the CYC1 UAS1 creates a new combination of regulatory elements that activate transcription synergistically.

作者信息

Sousa R, Arcangioli B

机构信息

Départment de Biologie Moléculaire, UA 1149 CNRS, Institut Pasteur, Paris, France.

出版信息

EMBO J. 1989 Jun;8(6):1801-8. doi: 10.1002/j.1460-2075.1989.tb03574.x.

DOI:10.1002/j.1460-2075.1989.tb03574.x
PMID:2548856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC401026/
Abstract

Dissection of the upstream activation site 1 (UAS1) of the yeast CYC1 gene showed that the A and B regions respond individually to regulation by the HAP1 protein, and that a point mutation in the B region converts this region to a translation upstream factor (TUF)-regulated element. Combinatorial analyses revealed that the transacting factors involved with these wild-type and mutant UAS1 target sites combine to activate transcription in a synergistic manner. Furthermore, combinations of heterologous factors, made possible by the point mutation, create a new specificity of regulation that differs from regulation by any one factor individually.

摘要

对酵母CYC1基因上游激活位点1（UAS1）的剖析表明，A区和B区分别对HAP1蛋白的调控作出反应，并且B区的一个点突变可将该区域转变为受翻译上游因子（TUF）调控的元件。组合分析显示，与这些野生型和突变型UAS1靶位点相关的反式作用因子协同激活转录。此外，点突变所促成的异源因子组合产生了一种新的调控特异性，这种特异性不同于任何单个因子的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/e9f9a2891441/emboj00130-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/73ba1ee0cd21/emboj00130-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/983db457699b/emboj00130-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/e9f9a2891441/emboj00130-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/73ba1ee0cd21/emboj00130-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/983db457699b/emboj00130-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb09/401026/e9f9a2891441/emboj00130-0170-a.jpg

相似文献

1
A point mutation in the CYC1 UAS1 creates a new combination of regulatory elements that activate transcription synergistically.CYC1 UAS1中的一个点突变产生了一种新的调控元件组合，可协同激活转录。
EMBO J. 1989 Jun;8(6):1801-8. doi: 10.1002/j.1460-2075.1989.tb03574.x.
2
Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.明显受调控的串联上游激活位点介导酿酒酵母CYC1基因的分解代谢物阻遏。
Cell. 1984 Feb;36(2):503-11. doi: 10.1016/0092-8674(84)90243-5.
3
Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene.酵母HAP1激活剂与因子RC2竞争结合CYC1基因的上游激活位点UAS1。
Cell. 1987 Apr 10;49(1):9-18. doi: 10.1016/0092-8674(87)90750-1.
4
Adjacent upstream activation sequence elements synergistically regulate transcription of ADH2 in Saccharomyces cerevisiae.相邻的上游激活序列元件协同调节酿酒酵母中ADH2的转录。
Mol Cell Biol. 1989 Jan;9(1):34-42. doi: 10.1128/mcb.9.1.34-42.1989.
5
Yeast HAP1 activator binds to two upstream activation sites of different sequence.酵母HAP1激活剂与两个不同序列的上游激活位点结合。
Cell. 1987 Apr 10;49(1):19-27. doi: 10.1016/0092-8674(87)90751-3.
6
Mutations that alter transcriptional activation but not DNA binding in the zinc finger of yeast activator HAPI.改变酵母激活因子HAPI锌指结构中转录激活作用但不改变DNA结合能力的突变
Nature. 1989 Nov 9;342(6246):200-3. doi: 10.1038/342200a0.
7
A single Saccharomyces cerevisiae upstream activation site (UAS1) has two distinct regions essential for its activity.单个酿酒酵母上游激活位点（UAS1）有两个对其活性至关重要的不同区域。
Mol Cell Biol. 1986 Dec;6(12):4690-6. doi: 10.1128/mcb.6.12.4690-4696.1986.
8
Designing zinc-finger ADR1 mutants with altered specificity of DNA binding to T in UAS1 sequences.设计在UAS1序列中与T的DNA结合特异性发生改变的锌指ADR1突变体。
Biochemistry. 1995 Mar 14;34(10):3222-30. doi: 10.1021/bi00010a011.
9
Characterization of a promoter mutation in the CYP3 gene of Saccharomyces cerevisiae which cancels regulation by Cyp1p (Hap1p) without affecting its binding site.酿酒酵母CYP3基因启动子突变的特征分析，该突变消除了Cyp1p（Hap1p）的调控作用，但不影响其结合位点。
Mol Gen Genet. 1996 Nov 27;253(1-2):103-10. doi: 10.1007/s004380050302.
10
HAP1 positive control mutants specific for one of two binding sites.针对两个结合位点之一的HAP1阳性对照突变体。
Genes Dev. 1992 Oct;6(10):2001-9. doi: 10.1101/gad.6.10.2001.

引用本文的文献

1
Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservation.酵母通用转录因子GFI：与DNA结合的序列要求及进化保守性
Nucleic Acids Res. 1990 May 11;18(9):2769-76. doi: 10.1093/nar/18.9.2769.
2
Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene.磷酸化作用会影响酵母RAP1蛋白与PGK基因上游激活序列的结合。
Nucleic Acids Res. 1990 Dec 25;18(24):7331-7. doi: 10.1093/nar/18.24.7331.
3
Expression of the gene encoding subunit II of yeast QH2: cytochrome c oxidoreductase is regulated by multiple factors.

本文引用的文献

1
Transcription and regulatory signals at the mating type locus in yeast.酵母交配型基因座的转录和调控信号。
Cell. 1984 Jul;37(3):969-78. doi: 10.1016/0092-8674(84)90431-8.
2
Yeast promoters: positive and negative elements.酵母启动子：正向和负向元件
Cell. 1984 Apr;36(4):799-800. doi: 10.1016/0092-8674(84)90028-x.
3
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
Curr Genet. 1990 Jun;17(6):459-64. doi: 10.1007/BF00313072.
4
Protein recognition sites in polyomavirus enhancer: formation of a novel site for NF-1 factor in an enhancer mutant and characterization of a site in the enhancer D domain.多瘤病毒增强子中的蛋白质识别位点：增强子突变体中NF-1因子新位点的形成及增强子D结构域中一个位点的特征分析
EMBO J. 1990 Mar;9(3):947-55. doi: 10.1002/j.1460-2075.1990.tb08193.x.
5
Regulation of gene expression by oxygen in Saccharomyces cerevisiae.酿酒酵母中氧气对基因表达的调控
Microbiol Rev. 1992 Mar;56(1):1-11. doi: 10.1128/mr.56.1.1-11.1992.
6
Regulation of the yeast RAD2 gene: DNA damage-dependent induction correlates with protein binding to regulatory sequences and their deletion influences survival.
Mol Gen Genet. 1992 Mar;232(2):247-56. doi: 10.1007/BF00280003.
7
Global regulation of mitochondrial biogenesis in Saccharomyces cerevisiae: ABF1 and CPF1 play opposite roles in regulating expression of the QCR8 gene, which encodes subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase.酿酒酵母线粒体生物合成的全局调控：ABF1和CPF1在调控QCR8基因的表达中发挥相反作用，该基因编码线粒体泛醇-细胞色素c氧化还原酶的亚基VIII。
Mol Cell Biol. 1992 Jun;12(6):2872-83. doi: 10.1128/mcb.12.6.2872-2883.1992.
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
4
Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.明显受调控的串联上游激活位点介导酿酒酵母CYC1基因的分解代谢物阻遏。
Cell. 1984 Feb;36(2):503-11. doi: 10.1016/0092-8674(84)90243-5.
5
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.用于研究酵母中克隆基因表达的酵母启动子和乳糖操纵子Z融合体。
Methods Enzymol. 1983;101:181-91. doi: 10.1016/0076-6879(83)01013-7.
6
Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.血红素通过一个上游激活位点调节酿酒酵母CYC1基因的转录。
Cell. 1983 Apr;32(4):1279-86. doi: 10.1016/0092-8674(83)90309-4.
7
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
8
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.一种用于定量蛋白质与特定DNA区域结合的凝胶电泳方法：应用于大肠杆菌乳糖操纵子调控系统的组分
Nucleic Acids Res. 1981 Jul 10;9(13):3047-60. doi: 10.1093/nar/9.13.3047.
9
Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.两种具有不同细胞特异性的不同增强子共存于多瘤病毒的调控区域。
Cell. 1984 Dec;39(3 Pt 2):653-62. doi: 10.1016/0092-8674(84)90472-0.
10
Analytical studies of 'mixed sequence' oligodeoxyribonucleotides synthesized by competitive coupling of either methyl- or beta-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, including 2'-deoxyinosine.对通过甲基或β-氰基乙基-N,N-二异丙基氨基亚磷酰胺试剂（包括2'-脱氧次黄苷）的竞争性偶联合成的“混合序列”寡脱氧核糖核苷酸的分析研究。
Nucleic Acids Res. 1985 Nov 25;13(22):8181-96. doi: 10.1093/nar/13.22.8181.