对通过甲基或β-氰基乙基-N,N-二异丙基氨基亚磷酰胺试剂（包括2'-脱氧次黄苷）的竞争性偶联合成的“混合序列”寡脱氧核糖核苷酸的分析研究。
Analytical studies of 'mixed sequence' oligodeoxyribonucleotides synthesized by competitive coupling of either methyl- or beta-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, including 2'-deoxyinosine.
作者信息
Zon G, Gallo K A, Samson C J, Shao K L, Summers M F, Byrd R A
出版信息
Nucleic Acids Res. 1985 Nov 25;13(22):8181-96. doi: 10.1093/nar/13.22.8181.
Abstract
High-performance liquid chromatography (HPLC) and 1H/31P nuclear magnetic resonance (NMR) spectroscopy were used to measure the molar ratio of oligodeoxyribonucleotide products in mixtures obtained with automated DNA synthesizers that employed competitive coupling of either standard methyl- or newer beta-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, which include deoxyinosine. Mixtures of these reagents when used as freshly prepared solutions afforded ratios of products that indicated negligibly small differences among the rates of the various competitive coupling reactions. However, studies of reagent stability in solution revealed that both types of the N-isobutyryl deoxyguanosine reagent decompose faster than their corresponding dA, dC, and dT phosphoramidites, which led to significantly lower proportions of dG-containing sequences. This problem was attenuated for the beta-cyanoethyl reagents due to their slower rate of decomposition.
摘要
高效液相色谱（HPLC）和1H/31P核磁共振（NMR）光谱法用于测量在使用标准甲基-或新型β-氰基乙基-N，N-二异丙基氨基亚磷酰胺试剂（包括脱氧肌苷）进行竞争性偶联的自动DNA合成仪所获得的混合物中寡脱氧核糖核苷酸产物的摩尔比。当将这些试剂的混合物用作新制备的溶液时，产物的比例表明各种竞争性偶联反应的速率之间的差异小到可以忽略不计。然而，对溶液中试剂稳定性的研究表明，两种类型的N-异丁酰基脱氧鸟苷试剂的分解速度都比其相应的dA、dC和dT亚磷酰胺快，这导致含dG序列的比例显著降低。由于β-氰基乙基试剂的分解速度较慢，这个问题得到了缓解。
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本文引用的文献
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