激动剂刺激后气管平滑肌中肌醇（1,4,5）三磷酸的质量变化
Mass changes of inositol(1,4,5)trisphosphate in trachealis muscle following agonist stimulation.
作者信息
Chilvers E R, Challiss R A, Barnes P J, Nahorski S R
机构信息
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
出版信息
Eur J Pharmacol. 1989 May 30;164(3):587-90. doi: 10.1016/0014-2999(89)90269-0.
Agonist-stimulated changes in D-Ins(1,4,5)P3 has been examined in tracheal smooth muscle using a specific radioreceptor assay utilizing the binding of [3H] or [32P]Ins(1,4,5)P3 to a bovine adrenal cortex preparation. Carbachol produced a maximal increase in Ins(1,4,5)P3 concentration after 5 s (basal: 12.9 +/- 0.8; stimulated: 27 +/- 1.5 pmol/mg protein) with an EC50 of 6.7 +/- 0.8 microM. With longer stimulation periods Ins(1,4,5)P3 rapidly returned to basal values by 30 s. This is the first report of mass measurements of Ins(1,4,5)P3 in smooth muscle.
利用[3H]或[32P]肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）与牛肾上腺皮质制剂的结合，通过特定的放射受体分析法，在气管平滑肌中研究了激动剂刺激引起的Ins(1,4,5)P3变化。卡巴胆碱在5秒后使Ins(1,4,5)P3浓度最大增加（基础值：12.9±0.8；刺激后：27±1.5皮摩尔/毫克蛋白），半数有效浓度（EC50）为6.7±0.8微摩尔。随着刺激时间延长，Ins(1,4,5)P3在30秒时迅速恢复到基础值。这是平滑肌中Ins(1,4,5)P3质量测量的首次报告。
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