Maden M, Ong D E, Summerbell D, Chytil F, Hirst E A
National Institute for Medical Research, Mill Hill, London, United Kingdom.
Dev Biol. 1989 Sep;135(1):124-32. doi: 10.1016/0012-1606(89)90163-2.
The distribution of cellular retinoic acid-binding protein (CRABP) in four stages of chick development is described using an affinity-purified antibody against rat CRABP. CRABP is the protein to which retinoic acid (RA) binds when it enters cells and may reflect the requirement of those cells for RA. We found several discrete cell populations which showed high levels of immunoreactivity. Some were in the neural tube such as the commissural neurons and the dorsal roof plate. Some were of neural crest origin such as the dorsal root ganglia, sensory axons, sympathetic ganglia, and enteric ganglia. The remaining populations were certain connective tissue cells, limb bud cells, and the myotome. These results suggest that certain organ systems, particularly the nervous system, have a requirement for RA during development and they may further our understanding of the teratogenic effects of retinoids on the embryo.
利用针对大鼠细胞视黄酸结合蛋白（CRABP）的亲和纯化抗体，描述了鸡发育四个阶段中CRABP的分布情况。CRABP是视黄酸（RA）进入细胞时与之结合的蛋白质，可能反映了这些细胞对RA的需求。我们发现了几个显示出高免疫反应性的离散细胞群体。一些位于神经管中，如连合神经元和背顶板。一些起源于神经嵴，如背根神经节、感觉轴突、交感神经节和肠神经节。其余的群体是某些结缔组织细胞、肢芽细胞和肌节。这些结果表明，某些器官系统，特别是神经系统，在发育过程中需要RA，它们可能会进一步加深我们对类维生素A对胚胎致畸作用的理解。
