Kato H
Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1989 May;64(3):318-27.
A NIH3T3-derived cell clone (NA7) in which human papillomavirus (HPV) 16 early region E6/E7 was inducible by dexamethasone (DXM) under the control of mouse mammary tumor virus long terminal repeat was established. A transforming function for HPV 16 E6/E7 region was analyzed by this established clone. Northern blot hybridization demonstrated that the increased expression of PCNA/cyclin and c-myc at the confluent state in accordance with the induced expression of E6/E7 region by DXM. Although complete transformation was not observed, the saturation density of NA7 was increased by the addition of DXM. The transfection assay using NA7 showed that adenovirus type 5 E1B (Ad5E1B) could cooperate with E6/E7. Furthermore, it was indicated that E7 could also cooperate with adenovirus type 5 E1B as well as with EJ-ras in transforming primary rat embryonal cells. However, E6/E7 could not cooperate with Ad12E1B in both cell systems. In this study, HPV 16 E6/E7 was assumed to have the growth-stimulatory activity in association with some cellular genes and transforming activity by cooperation with some transforming genes. These results suggest that E6/E7 seems to play a major role in the process of human cervical cell carcinogenesis.
建立了一种源自NIH3T3的细胞克隆（NA7），在小鼠乳腺肿瘤病毒长末端重复序列的控制下，人乳头瘤病毒（HPV）16早期区域E6/E7可被地塞米松（DXM）诱导表达。利用该已建立的克隆分析HPV 16 E6/E7区域的转化功能。Northern印迹杂交显示，在汇合状态下，PCNA/细胞周期蛋白和c-myc的表达增加，这与DXM诱导E6/E7区域的表达一致。尽管未观察到完全转化，但添加DXM可增加NA7的饱和密度。使用NA7的转染试验表明，5型腺病毒E1B（Ad5E1B）可与E6/E7协同作用。此外，研究表明E7在转化原代大鼠胚胎细胞时也可与5型腺病毒E1B以及EJ-ras协同作用。然而，在这两种细胞系统中，E6/E7均不能与12型腺病毒E1B协同作用。在本研究中，推测HPV 16 E6/E7与某些细胞基因相关联具有生长刺激活性，并通过与某些转化基因协同作用具有转化活性。这些结果表明，E6/E7似乎在人宫颈细胞癌变过程中起主要作用。
