Singh Rashmi, Cabrera Miguel L, Radcliffe David E, Zhang Hao, Huang Qingguo
Department of Crop and Soil Sciences, University of Georgia, Griffin, GA 30223, USA.
Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USA.
Environ Pollut. 2015 Feb;197:28-35. doi: 10.1016/j.envpol.2014.11.023. Epub 2014 Dec 6.
It is known that 17β-estradiol (E2) can be transformed by reactions mediated by some oxidoreductases such as laccase in water. Whether or how such reactions can happen in soil is however unknown although they may significantly impact the environmental fate of E2 that is introduced to soil by land application of animal wastes. We herein studied the reaction of E2 in a model soil mediated by laccase, and found that the reaction behaviors differ significantly from those in water partly because of the dramatic difference in laccase stability. We also examined E2 transformation in soil using (14)C-labeling in combination with soil organic matter extraction and size exclusion chromatography, which indicated that applied (14)C radioactivity was preferably bound to humic acids. The study provides useful information for understanding the environmental fate of E2 and for developing a novel soil remediation strategy via enzyme-enhanced humification reactions.
已知17β-雌二醇（E2）可通过水中某些氧化还原酶（如漆酶）介导的反应进行转化。然而，尽管这些反应可能会显著影响通过动物粪便土地施用进入土壤的E2的环境归宿，但此类反应在土壤中是否会发生以及如何发生尚不清楚。我们在此研究了漆酶介导的E2在模拟土壤中的反应，发现反应行为与在水中的反应行为有显著差异，部分原因是漆酶稳定性存在巨大差异。我们还结合土壤有机质提取和尺寸排阻色谱法，使用（14）C标记研究了土壤中的E2转化，结果表明施加的（14）C放射性物质更倾向于与腐殖酸结合。该研究为理解E2的环境归宿以及通过酶促腐殖化反应开发新型土壤修复策略提供了有用信息。
