Yang Qainhui, Dong Wenxiu, Ye Zesen, Wei Zebin, Wu Qitang, Qiu Rongliang, Li Mi, Chen Yangmei
College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China.
Center for Renewable Carbon, School of Natural Resources, University of Tennessee, Knoxville, TN, 37996, USA.
Sci Rep. 2025 Apr 28;15(1):14765. doi: 10.1038/s41598-025-98586-9.
Hazardous 17β-estradiol (E2) with a higher estrogen effect, has attracted the attention of many scholars. How to transform and remove E2 in the environment becomes a research hotspot. This paper systematically explores the impacts of environmental elements like temperature, pH value, laccase concentration, humic acid concentration, and the type and concentration of metal ions on the laccase-catalyzed oxidation process of E2. The results show that the optimal environmental conditions for laccase-catalyzed oxidation of E2 are temperatures of 25 °C to 30 °C, pH values of 4 to 5, laccase concentration of 30 unit·L, no addition of humic acid, and the addition of Cu at a density of 1 mmol·L. The conversion rate of E2 by laccase remained above 70%. The coupling products were formed through covalent coupling mechanisms mediated by functional groups such as C-C and C-O-C, with the main product being E2 dimers. E2 underwent chain extension polymerization during the reaction. Laccase has the potential to remediate E2 contaminated environment in a green and efficient manner.
具有较高雌激素效应的有害17β-雌二醇（E2）引起了众多学者的关注。如何在环境中转化和去除E2成为研究热点。本文系统地探讨了温度、pH值、漆酶浓度、腐殖酸浓度以及金属离子的种类和浓度等环境因素对漆酶催化氧化E2过程的影响。结果表明，漆酶催化氧化E2的最佳环境条件为温度25℃至30℃、pH值4至5、漆酶浓度30单位·L、不添加腐殖酸以及添加密度为1 mmol·L的Cu。漆酶对E2的转化率保持在70%以上。偶联产物通过C-C和C-O-C等官能团介导的共价偶联机制形成，主要产物为E2二聚体。E2在反应过程中发生链延伸聚合。漆酶具有以绿色高效的方式修复受E2污染环境的潜力。
