Sillence M N, Etherton T D
Pennsylvania State University, University Park 16802.
J Anim Sci. 1989 Jul;67(7):1740-3. doi: 10.2527/jas1989.6771740x.
This study was conducted to examine serum cortisol concentrations and adrenal cortisol output in pigs treated chronically with recombinant pGH (rpGH) at a maximally effective anabolic dose. Recombinant pGH (140 micrograms/kg body weight) was administered daily to eight barrows for 77 d. At slaughter, adrenal glands were removed, weighed and sliced; slices of fresh adrenal tissue were incubated for 1 h in the presence or absence of ACTH. Recombinant pGH increased adrenal weight by 39%. This change was accompanied by an inversely proportional reduction of in vitro cortisol output per gram of tissue, with the net result that total cortisol output per adrenal per kilogram of BW was unaltered, as was cortisol output in the presence of ACTH. Serum cortisol concentrations were measured in 10 barrows fitted with femoral artery catheters and treated daily with 0 or 140 micrograms rpGH/kg BW for 8 d. Basal and ACTH-stimulated cortisol concentrations were not altered by rpGH treatment. These results do not support our earlier speculation that a pGH-dependent increase in adrenal weight is associated with a chronic increase in adrenal activity, but rather demonstrate that corticosteroid output is tightly regulated and remains constant despite a marked increase in the size of the adrenal glands.
本研究旨在检测以最大有效合成代谢剂量长期接受重组猪生长激素（rpGH）治疗的猪的血清皮质醇浓度和肾上腺皮质醇分泌量。将重组猪生长激素（140微克/千克体重）每日注射给8头公猪，持续77天。屠宰时，取出肾上腺，称重并切片；新鲜肾上腺组织切片在有或无促肾上腺皮质激素（ACTH）的情况下孵育1小时。重组猪生长激素使肾上腺重量增加了39%。这种变化伴随着每克组织体外皮质醇分泌量成反比减少，最终结果是每千克体重每个肾上腺的皮质醇总分泌量未改变，在有促肾上腺皮质激素的情况下皮质醇分泌量也未改变。对10头装有股动脉导管且每日接受0或140微克/千克体重重组猪生长激素治疗8天的公猪测量血清皮质醇浓度。重组猪生长激素治疗未改变基础和促肾上腺皮质激素刺激后的皮质醇浓度。这些结果不支持我们早期的推测，即猪生长激素依赖性肾上腺重量增加与肾上腺活动的慢性增加有关，而是表明尽管肾上腺大小显著增加，但皮质类固醇分泌受到严格调节并保持恒定。
