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来自嗜热嗜酸硫化叶菌的糖原结合型多聚磷酸激酶。

Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius.

作者信息

Skórko R, Osipiuk J, Stetter K O

机构信息

Lehrstuhl für Mikrobiologie, Universität Regensburg, Federal Republic of Germany.

出版信息

J Bacteriol. 1989 Sep;171(9):5162-4. doi: 10.1128/jb.171.9.5162-5164.1989.

DOI:10.1128/jb.171.9.5162-5164.1989
PMID:2549015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210331/
Abstract

Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein from the glycogen complex migrated in a sodium dodecyl sulfate-polyacrylamide gel as a 57-kilodalton protein band; after isoelectric focusing it separated into several spots in the pH range of 5.6 to 6.7.

摘要

通过在氯化铯中进行等密度离心，从嗜酸热硫化叶菌的粗提物中分离出了与糖原结合的多聚磷酸激酶。二价阳离子（锰离子比镁离子更有效）能刺激该反应。该酶的活性不需要组蛋白的存在；它受到磷酸盐的强烈抑制，受到氟化物的轻微抑制。糖原复合物中的蛋白质在十二烷基硫酸钠-聚丙烯酰胺凝胶中迁移时呈现为一条57千道尔顿的蛋白带；等电聚焦后，它在5.6至6.7的pH范围内分离成几个斑点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/5cabb2e21fc2/jbacter00175-0648-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/46e4be293158/jbacter00175-0647-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/8a6c8b60e2d1/jbacter00175-0647-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/5cabb2e21fc2/jbacter00175-0648-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/46e4be293158/jbacter00175-0647-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/8a6c8b60e2d1/jbacter00175-0647-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/228b/210331/5cabb2e21fc2/jbacter00175-0648-a.jpg

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