Doğaç Ersin, Kandemir İrfan, Taşkın Vatan
Department of Biology, Faculty of Science, Muğla Sıtkı Kocman University, Kotekli, Muğla, Turkey.
Department of Biology, Faculty of Science, Ankara University, Beşevler, Ankara, Turkey.
Pest Manag Sci. 2015 Nov;71(11):1529-39. doi: 10.1002/ps.3958. Epub 2015 Jan 13.
In the Mediterranean basin, organophosphate (OP) insecticides have been used intensively to control olive fly populations. Acetylcholinesterase (Ace) is the molecular target of OP insecticides, and three resistance-associated mutations that confer different levels of OP insensitivity have been identified. In this study, genotypes of olive fly Ace were determined in field-collected populations from broad geographical areas in Turkey. In addition, the levels of asymmetry of wing and leg characters were compared in these populations.
Our study revealed the existence of a genetically smooth stratification pattern in OP resistance allele distribution in the olive fly populations of Turkey. In contrast to earlier findings, the frequency of Δ3Q was found to be lower in the Aegean region, where the populations have been subjected to high selection pressure. Results based on the morphological differences among the samples revealed a similar pattern for both sides and did not demonstrate a clear separation.
The frequencies and geographic range of resistance alleles indicate that they were selected in the Aegean coast of Turkey and then spread westward towards Europe. One possible explanation for the absence of morphological asymmetry in olive fly samples might be the presence of modifier allele(s) that compensate for the increase in asymmetry.
在地中海盆地，有机磷（OP）杀虫剂已被大量用于控制橄榄果蝇种群。乙酰胆碱酯酶（Ace）是OP杀虫剂的分子靶点，已鉴定出三种与抗性相关的突变，这些突变赋予了不同程度的OP不敏感性。在本研究中，测定了来自土耳其广泛地理区域的田间采集种群中橄榄果蝇Ace的基因型。此外，还比较了这些种群中翅和腿特征的不对称程度。
我们的研究揭示了土耳其橄榄果蝇种群中OP抗性等位基因分布存在遗传上平滑的分层模式。与早期研究结果相反，在遭受高选择压力的爱琴海地区，发现Δ3Q的频率较低。基于样本形态差异的结果显示两侧存在相似模式，未显示出明显的分离。
抗性等位基因的频率和地理范围表明它们在土耳其爱琴海沿岸被选择，然后向西扩散到欧洲。橄榄果蝇样本中不存在形态不对称的一个可能解释可能是存在补偿不对称性增加的修饰等位基因。
