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涂覆有功能化碳纳米管的钛——一种有前途的新型材料,可作为可植入骨科电子设备用于生物医学应用。

Titanium coated with functionalized carbon nanotubes--a promising novel material for biomedical application as an implantable orthopaedic electronic device.

作者信息

Przekora Agata, Benko Aleksandra, Nocun Marek, Wyrwa Jan, Blazewicz Marta, Ginalska Grazyna

机构信息

Department of Biochemistry and Biotechnology, Medical University of Lublin, Faculty of Pharmacy with Medical Analytics Division, Chodzki 1, 20-093 Lublin, Poland.

Faculty of Materials Science and Ceramics, AGH-Univ. of Science and Technology, A. Mickiewicz 30 Ave., 30-059 Cracow, Poland.

出版信息

Mater Sci Eng C Mater Biol Appl. 2014 Dec;45:287-96. doi: 10.1016/j.msec.2014.09.025. Epub 2014 Sep 16.

Abstract

The aim of the study was to fabricate titanium (Ti) material coated with functionalized carbon nanotubes (f-CNTs) that would have potential medical application in orthopaedics as an implantable electronic device. The novel biomedical material (Ti-CNTs-H2O) would possess specific set of properties, such as: electrical conductivity, non-toxicity, and ability to inhibit connective tissue cell growth and proliferation protecting the Ti-CNTs-H2O surface against covering by cells. The novel material was obtained via an electrophoretic deposition of CNTs-H2O on the Ti surface. Then, physicochemical, electrical, and biological properties were evaluated. Electrical property evaluation revealed that a Ti-CNTs-H2O material is highly conductive and X-ray photoelectron spectroscopy analysis demonstrated that there are mainly COOH groups on the Ti-CNTs-H2O surface that are found to inhibit cell growth. Biological properties were assessed using normal human foetal osteoblast cell line (hFOB 1.19). Conducted cytotoxicity tests and live/dead fluorescent staining demonstrated that Ti-CNTs-H2O does not exert toxic effect on hFOB cells. Moreover, fluorescence laser scanning microscope observation demonstrated that Ti-CNTs-H2O surface retards to a great extent cell proliferation. The study resulted in successful fabrication of highly conductive, non-toxic Ti-CNTs-H2O material that possesses ability to inhibit osteoblast proliferation and thus has a great potential as an orthopaedic implantable electronic device.

摘要

本研究的目的是制造一种涂覆有功能化碳纳米管(f-CNTs)的钛(Ti)材料,该材料作为可植入电子设备在骨科领域具有潜在的医学应用价值。这种新型生物医学材料(Ti-CNTs-H₂O)将具备一系列特定的性能,例如:导电性、无毒性以及抑制结缔组织细胞生长和增殖的能力,从而保护Ti-CNTs-H₂O表面不被细胞覆盖。通过在Ti表面电泳沉积CNTs-H₂O获得了这种新型材料。然后,对其物理化学、电学和生物学性能进行了评估。电学性能评估表明,Ti-CNTs-H₂O材料具有高导电性,X射线光电子能谱分析表明,Ti-CNTs-H₂O表面主要存在COOH基团,这些基团被发现能够抑制细胞生长。使用正常人胎儿成骨细胞系(hFOB 1.19)评估了生物学性能。进行的细胞毒性测试和活/死荧光染色表明,Ti-CNTs-H₂O对hFOB细胞没有毒性作用。此外,荧光激光扫描显微镜观察表明,Ti-CNTs-H₂O表面在很大程度上抑制了细胞增殖。该研究成功制造出了具有高导电性、无毒性且具备抑制成骨细胞增殖能力的Ti-CNTs-H₂O材料,因此作为骨科可植入电子设备具有巨大潜力。

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