Terada Michiko, Abe Shigeaki, Akasaka Tsukasa, Uo Motohiro, Kitagawa Yoshimasa, Watari Fumio
Oral Diagnosis and Oral Medicine, Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University, Kitaku, Sapporo, Japan.
Biomed Mater Eng. 2009;19(1):45-52. doi: 10.3233/BME-2009-0562.
Carbon nanotubes (CNTs) have excellent chemical durability, mechanical strength and electrical properties. Therefore, there is interest in CNTs for not only electrical and mechanical applications, but also biological and medical applications. We coated titanium, a common material for dental implants, with multiwalled carbon nanotubes (MWCNTs). First, titanium was aminated and covered with collagen. Then, the carboxylated MWCNTs were coated onto the collagen attached to the titanium plate. The collagen-coated titanium plate had a homogeneous MWCNT coating, which showed strong attachment to the titanium surface as a thin layer. The surface roughness was significantly increased with the MWCNT coating. MC3T3-E1 cells were cultured on the MWCNT-coated Ti plate, and showed good cell proliferation and strong cell adhesion. Therefore, the MWCNT coating for titanium could be useful for improvement of cell adhesion on titanium implants.
碳纳米管（CNTs）具有出色的化学耐久性、机械强度和电学性能。因此，人们不仅对碳纳米管在电气和机械应用方面感兴趣，而且对其在生物和医学应用方面也感兴趣。我们用多壁碳纳米管（MWCNTs）包覆了牙科植入物常用材料钛。首先，对钛进行胺化处理并覆盖胶原蛋白。然后，将羧基化的多壁碳纳米管包覆在附着于钛板的胶原蛋白上。包覆胶原蛋白的钛板有一层均匀的多壁碳纳米管涂层，该涂层作为薄层牢固地附着在钛表面。多壁碳纳米管涂层使表面粗糙度显著增加。将MC3T3-E1细胞培养在包覆多壁碳纳米管的钛板上，细胞表现出良好的增殖和较强的黏附能力。因此，用于钛的多壁碳纳米管涂层可能有助于改善钛植入物上的细胞黏附。
