Kessous-Elbaz A, Pelletier M, Cohen E A, Langelier Y
Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, Québec, Canada.
J Gen Virol. 1989 Aug;70 ( Pt 8):2171-7. doi: 10.1099/0022-1317-70-8-2171.
Cotransfection experiments have been carried out using recombinant plasmids pAG60, conferring resistance to antibiotic G418, and pXho3 which contains the left end subfragment (map coordinates 0.583 to 0.596) of the transforming herpes simplex virus type 2 BglII N DNA fragment and encodes the 36K polypeptide associated with the viral ribonucleotide reductase activity. Several NIH 3T3 cell clones resistant to G418 and having morphological changes commonly observed for transformed NIH 3T3 cells were isolated and examined for the presence and stable retention of the viral sequences. Seven of the clones that retained the transfected viral sequences were analysed for the expression of the 36K polypeptide and the tumorigenic phenotype. The results gathered from these studies show that neither the retention of the viral DNA nor the expression of the 36K polypeptide correlated with tumorigenic conversion of these cells.
共转染实验使用了赋予抗生素G418抗性的重组质粒pAG60和含有转化型单纯疱疹病毒2型BglII N DNA片段左端亚片段（图谱坐标0.583至0.596）并编码与病毒核糖核苷酸还原酶活性相关的36K多肽的pXho3进行。分离出几个对G418有抗性且具有转化的NIH 3T3细胞常见形态变化的NIH 3T3细胞克隆，并检测病毒序列的存在和稳定保留情况。对七个保留了转染病毒序列的克隆进行了36K多肽表达和致瘤表型分析。这些研究收集的结果表明，病毒DNA的保留或36K多肽的表达均与这些细胞的致瘤转化无关。
