脂解剂和抗脂解剂对正常和糖尿病大鼠分离脂肪细胞甘油和游离脂肪酸释放的影响。

Effects of lipolytic and antilipolytic agents on glycerol and free fatty acid release from isolated adipocytes of normal and diabetic rats.

作者信息

Kamei I, Hotta N, Kakuta H, Kimura M, Fukasawa H, Koh N, Sakamoto N

出版信息

Nagoya J Med Sci. 1989 Mar;51(1-4):17-23.

Abstract

Isolated adipocytes from severely diabetic rats exhibited hypersensitivity to epinephrine at low concentrations (0.05-0.1 microM) on lipolysis, compared with isolated adipocytes from normal and mildly diabetic rats. Hypersensitivity to dibutyryl cyclic AMP and theophylline at concentrations from 0.05 to 0.50 mM was not observed in adipocytes of severely diabetic rats. Insulin could not exert an inhibitory effect on epinephrine-induced lipolysis in adipocytes of severely diabetic rats. In isolated adipocytes from normal rats, hyperosmolarity due to the combination of 50 mM glucose and 100 mM sodium chloride only had an inhibitory effect on 0.25 microM epinephrine-induced lipolysis. Ten mM beta-hydroxybutyrate did not inhibit lipolysis caused by epinephrine although any lipolysis stimulated by epinephrine, dibutyryl cyclic AMP and theophylline was inhibited by insulin. Our present findings may partly explain insulin resistance in the severely diabetic state and the pathogenesis of the absence of ketosis in hyperglycemic hyperosmolar conditions.

摘要

与正常和轻度糖尿病大鼠分离的脂肪细胞相比，严重糖尿病大鼠分离的脂肪细胞在低浓度（0.05 - 0.1微摩尔）肾上腺素作用下对脂解表现出超敏反应。在严重糖尿病大鼠的脂肪细胞中未观察到对浓度为0.05至0.50毫摩尔的二丁酰环磷酸腺苷和茶碱的超敏反应。胰岛素对严重糖尿病大鼠脂肪细胞中肾上腺素诱导的脂解没有抑制作用。在正常大鼠分离的脂肪细胞中，50毫摩尔葡萄糖和100毫摩尔氯化钠组合导致的高渗仅对0.25微摩尔肾上腺素诱导的脂解有抑制作用。10毫摩尔β-羟基丁酸并不抑制肾上腺素引起的脂解，尽管胰岛素可抑制肾上腺素、二丁酰环磷酸腺苷和茶碱刺激的任何脂解。我们目前的发现可能部分解释了严重糖尿病状态下的胰岛素抵抗以及高血糖高渗状态下无酮症的发病机制。