Ruth P, Röhrkasten A, Biel M, Bosse E, Regulla S, Meyer H E, Flockerzi V, Hofmann F
Institut für Physiologische Chemie, Medizinische Fakultät, Homburg/Saar, Federal Republic of Germany.
Science. 1989 Sep 8;245(4922):1115-8. doi: 10.1126/science.2549640.
Complementary DNAs for the beta subunit of the dihydropyridine-sensitive calcium channel of rabbit skeletal muscle were isolated on the basis of peptide sequences derived from the purified protein. The deduced primary structure is without homology to other known protein sequences and is consistent with the beta subunit being a peripheral membrane protein associated with the cytoplasmic aspect of the sarcolemma. The protein contains sites that might be expected to be preferentially phosphorylated by protein kinase C and guanosine 3',5'-monophosphate-dependent protein kinase. A messenger RNA for this protein appears to be expressed in brain.
基于从纯化蛋白获得的肽序列，分离出了兔骨骼肌二氢吡啶敏感钙通道β亚基的互补DNA。推导的一级结构与其他已知蛋白质序列无同源性，这与β亚基是一种与肌膜胞质面相关的外周膜蛋白相一致。该蛋白质含有可能被蛋白激酶C和鸟苷3',5'-单磷酸依赖性蛋白激酶优先磷酸化的位点。编码这种蛋白质的信使核糖核酸似乎在脑中表达。
