球体培养促进角膜基质细胞的干性。

Spheroid cultures promote the stemness of corneal stromal cells.

作者信息

Li Hongyang, Dai Ying, Shu Jianchang, Yu Rongjie, Guo Yonglong, Chen Jiansu

机构信息

Ophthalmology Department, First Affiliated Hospital of Jinan University, Guangzhou, China; Ophthalmology Department, Guangzhou Red Cross Hospital Affiliated to Medical College of Jinan University, Guangzhou, China.

Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, China.

出版信息

Tissue Cell. 2015 Feb;47(1):39-48. doi: 10.1016/j.tice.2014.10.008. Epub 2014 Nov 7.

DOI:10.1016/j.tice.2014.10.008

PMID:25497331

Abstract

Several culture methods generated spheroids of rabbit and mouse corneal stromal cells (CSCs) in vitro. In this study, rabbit CSC spheroids were positively expressed the mesenchymal and stem cell phenotypes, which contained immunopositive for vimentin (a mesenchymal cell marker) and CD34 (a stem cell marker), as well as mRNA expression of nestin (a neural stem cell marker) and Nanog (a stem cell marker), in suspension or adherent cultures that were induced by methylcellulose, a rotary cell culture system (RCCS) or reprogramming proteins and VPA. Mouse CSCs showed poor growth and hardly formed spheroids after treatment with methylcellulose or reprogramming proteins and VPA. Our work has laid a promising foundation to elucidate CSCs and the further use of CSC spheroids for reprogramming, bioprinting and tissue engineering.

摘要

几种培养方法可在体外生成兔和小鼠角膜基质细胞（CSCs）的球体。在本研究中，兔CSC球体在甲基纤维素、旋转细胞培养系统（RCCS）或重编程蛋白及丙戊酸诱导的悬浮或贴壁培养中，阳性表达间充质和干细胞表型，其中波形蛋白（一种间充质细胞标志物）和CD34（一种干细胞标志物）免疫阳性，以及巢蛋白（一种神经干细胞标志物）和Nanog（一种干细胞标志物）的mRNA表达。用甲基纤维素或重编程蛋白及丙戊酸处理后，小鼠CSCs生长不佳，几乎不形成球体。我们的工作为阐明CSCs以及进一步将CSC球体用于重编程、生物打印和组织工程奠定了有前景的基础。