Martins Alessandro F, Facchi Suelen P, Follmann Heveline D M, Gerola Adriana P, Rubira Adley F, Muniz Edvani C
Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brazil; Grupo de Materiais Poliméricos e Compósitos (GMPC), Departamento de Química, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brazil.
Carbohydr Res. 2015 Jan 30;402:252-60. doi: 10.1016/j.carres.2014.10.002. Epub 2014 Oct 20.
Different methodologies were employed in this study to synthesize N,N,N-trimethyl chitosan salts (TMC). TMC free of O-methylation and with partial O-methylation were obtained and characterized through 1H nuclear magnetic resonance, wide angle X-ray scattering, scanning electron microscopy coupled with X-ray energy dispersive spectroscopy, and thermogravimetric analysis. It was verified that the dialysis process allowed the removal of 'surface ion pairs' on TMC salt structure, increasing the mobility of TMC chains. The surface ion pairs considerably increased the material crystallinity, this property being independent of the used synthesis methodology. Biological tests showed that after dialysis, TMC salts free of 'surface ion pairs' kill Escherichia coli in only 6h of incubation. So, the increase in the mobility of dialyzed TMC chains allowed a strong interaction with the cell envelope and the good bactericidal activity of TMC was enhanced.
本研究采用不同方法合成N，N，N-三甲基壳聚糖盐（TMC）。通过1H核磁共振、广角X射线散射、扫描电子显微镜结合X射线能量色散光谱以及热重分析，获得了无O-甲基化和部分O-甲基化的TMC并对其进行了表征。证实透析过程可去除TMC盐结构上的“表面离子对”，增加TMC链的流动性。表面离子对显著提高了材料的结晶度，此性质与所用的合成方法无关。生物学测试表明，透析后，不含“表面离子对”的TMC盐在孵育仅6小时后就能杀死大肠杆菌。因此，透析后TMC链流动性的增加使其与细胞膜有强烈相互作用，增强了TMC良好的杀菌活性。
