Wei Y, Zheng D, Li X, Zhou W, Qian Y, Ming C, Shi B
Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
Transplant Proc. 2014 Dec;46(10):3641-6. doi: 10.1016/j.transproceed.2014.09.093.
PUVA-SP DCs are immature dendritic cells (imDCs) that have taken up donor splenic lymphocytes treated with 8-methoxypsoralen and ultraviolet A light (PUVA-SPs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient imDCs, and infusion of PUVA-SP DCs can induce CD4(+)CD25(high)Foxp3(+) regulatory T cells (Treg). However, the regulatory effect of PUVA-SP DCs on B cells is poorly understood. In this study, we compared PUVA-SP DCs with imDCs in terms of lipopolysaccharide (LPS) responsiveness and flow cytometric analysis of interleukin (IL) 10 expression in splenic CD19(+)B cells. Our results demonstrate that PUVA-SP DCs can suppress subsequent LPS-induced DC maturation and that infusion of PUVA-SP DCs, in the absence of an immunosuppressant, significantly promotes skin allograft survival. This effect was associated with up-regulation of circulating regulatory B cells exhibiting preferential IL-10 secretion. Our results suggest that effective treatments involving infusion of PUVA-SP DCs is likely related to the modulation not only of T-cell and Treg functions but also of B-cell and regulatory B-cell (Breg) functions.
补骨脂素-紫外线A光处理的供体脾淋巴细胞负载的树突状细胞(PUVA-SP DCs)是已摄取经8-甲氧基补骨脂素和紫外线A光(PUVA-SP)处理的供体脾淋巴细胞的未成熟树突状细胞(imDCs)。供体PUVA-SP的吞噬作用不会刺激受体imDCs的表型成熟,输注PUVA-SP DCs可诱导CD4(+)CD25(high)Foxp3(+)调节性T细胞(Treg)。然而,PUVA-SP DCs对B细胞的调节作用尚不清楚。在本研究中,我们比较了PUVA-SP DCs和imDCs在脂多糖(LPS)反应性以及脾CD19(+)B细胞中白细胞介素(IL)10表达的流式细胞术分析方面的差异。我们的结果表明,PUVA-SP DCs可抑制随后LPS诱导的DC成熟,并且在没有免疫抑制剂的情况下输注PUVA-SP DCs可显著促进皮肤同种异体移植的存活。这种效应与循环调节性B细胞的上调有关,这些调节性B细胞表现出优先分泌IL-10。我们的结果表明,涉及输注PUVA-SP DCs的有效治疗可能不仅与T细胞和Treg功能的调节有关,还与B细胞和调节性B细胞(Breg)功能的调节有关。